Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Thalamic Subregion Alterations and Short-Chain Fatty Acids in Schizophrenia and Ultra-High-Risk Individuals: A Cross-Sectional Study.

Alpha psychiatry·2026

Same author

Highly Selective Construction of D<sub>2</sub>-Symmetric Chiral Carbon Nanorings and the Diverse Assembly With Fullerenes.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

Tachyarrhythmia is a prognostic factor for severity and mortality in patients with Severe Fever with Thrombocytopenia Syndrome.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026

Same author

Author Correction: Acoustic metamaterials-driven transdermal drug delivery for rapid and on-demand management of acute disease.

Nature communications·2026

Same author

Integrated microfluidic biosensors: shaping the future of quantitative life sciences and on-chip molecular diagnostics.

Lab on a chip·2026

Same author

Rare ectopic colloid cyst of the pituitary stalk presenting with short stature and diabetes insipidus.

Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery·2026

Search research articles

Related Experiment Video

Updated: Jul 7, 2025

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Cellular immunity analysis by a modular acoustofluidic platform: CIAMAP.

Ruoyu Zhong¹, Matthew Sullivan², Neil Upreti³

¹Thomas Lord Department of Mechanical Engineering & Materials Science, Duke University, Durham, NC 27708, USA.

Science Advances

|December 22, 2023

Summary

This summary is machine-generated.

A new acoustofluidic platform, CIAMAP, efficiently pairs single cells for immunotherapy research. It enables precise analysis of immune responses and intercellular communication, overcoming limitations of existing methods.

More Related Videos

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Related Experiment Videos

Last Updated: Jul 7, 2025

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Single-cell analysis is crucial for understanding immunotherapy, neuroinflammation, and neurodegenerative diseases.
Current single-cell pairing platforms suffer from low efficiency, complex operations, and single-use limitations.

Purpose of the Study:

To develop a novel platform for multiparametric cellular immunity analysis.
To improve the efficiency and functionality of single-cell pairing for immunological studies.

Main Methods:

Development of a modular acoustofluidic platform named CIAMAP.
Utilizing CIAMAP for efficient sorting and collection of effector-target cell pairs (NK92-K562).
Real-time monitoring of immunological response formation, alongside transcriptional and protein expression analyses.

Main Results:

CIAMAP demonstrated efficient sorting and collection of effector-target cell pairs.
The platform enabled real-time monitoring of immune response dynamics.
Transcriptional and protein analyses revealed pathways mediating effector cytotoxicity and doxorubicin's synergistic effects.

Conclusions:

CIAMAP offers a promising solution for high-throughput, quantitative single-cell coculture.
The platform advances the understanding of intercellular communication and empowers immunotherapy through precision analysis of immunological synapses.