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Updated: Jul 7, 2025
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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
Published on: June 23, 2022
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Revealing protein trafficking by proximity labeling-based proteomics
1School of Pharmaceutical Sciences, Tsinghua University, Beijing, China; Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
Bioorganic Chemistry
|December 22, 2023
Summary
Proximity labeling (PL) maps protein trafficking within and between cells. This review highlights advanced PL methods for understanding dynamic proteome movement and communication.
Area of Science:
- Cell Biology
- Proteomics
- Biochemistry
Background:
- Protein trafficking is crucial for cellular and intercellular functions.
- Proximity labeling (PL) offers a powerful method to study protein localization and interactions.
- Understanding proteome dynamics is key to deciphering cellular mechanisms.
Approach:
- This review focuses on recent advancements in proximity labeling (PL) technologies.
- Emphasis is placed on the label-and-fractionation approach and TransitID for proteome mapping.
- These methods enable the capture of precise subcellular proteome snapshots.
Key Points:
- PL technology utilizes promiscuous enzymes to tag proteins at specific cellular locations.
- Mass spectrometry-based proteomics identifies proximity-labeled endogenous proteins.
- Advanced PL methods facilitate the study of dynamic intracellular protein trafficking between organelles.
Conclusions:
- PL methodologies provide powerful tools for mapping proteome trafficking.
- These techniques illuminate both intracellular organelle-to-organelle communication and intercellular protein exchange.
- Further exploration of PL advancements will deepen our understanding of complex biological networks.
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