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Urine Sampling Protocol Recommendations for Reliable Determination of Total Urinary Luteinizing Hormone
And Demir1, Adem Aydin2, Atilla Büyükgebiz3
1Pediatric Research Center, New Children's Hospital, University of Helsinki and Helsinki University Hospital, 00290 Helsinki, Finland.
Insights
Urinary luteinizing hormone (U-LH) levels in children show random daily variations, especially in adolescents. Collecting multiple morning urine samples over three days is recommended for accurate U-LH measurement in pediatric studies.
Area of Science:
- Pediatric Endocrinology
- Reproductive Endocrinology
- Biomarker Analysis
Background:
- Accurate measurement of urinary luteinizing hormone (U-LH) is crucial for pediatric endocrine research.
- Day-to-day variability in U-LH can impact the reliability of study findings.
- Existing methods may not fully account for compositional changes in pediatric urine samples.
Purpose of the Study:
- To investigate day-to-day variations in urinary luteinizing hormone (U-LH) concentrations in children and adolescents.
- To identify potential methods for minimizing or correcting these variations.
- To assess the reliability of U-LH measurements over consecutive days in a pediatric cohort.
Main Methods:
- Collection of daytime and evening urine samples from 95 children and adolescents (ages 5-17) over three consecutive days.
- Determination of urinary luteinizing hormone (U-LH) concentrations using immunoassay techniques.
- Calculation of net inter-assay coefficient of variation (CV%) to quantify U-LH variability.
Main Results:
- No consistent day-to-day differences in U-LH levels were observed across the study cohort.
- Significant random variations in U-LH were noted, particularly in adolescents aged 13 and older.
- High inter-assay CV% for U-LH changes over three days (21.6%-28.0%) was independent of sex, collection time, or U-LH level.
Conclusions:
- Current U-LH measurement protocols exhibit considerable variability in pediatric populations.
- Multiple first-morning voided urine samples collected over at least three consecutive days are recommended as an interim measure for reliable U-LH assessment.
- Further development of standardized protocols or correction methods for urine composition is needed for accurate pediatric U-LH determination.
Abstract:
This study investigates day-to-day variations in urinary luteinizing hormone (U-LH) concentrations in children, focusing on potential minimization or correction methods. 95 children and adolescents (51 boys, 44 girls, ages 5-17) provided daytime and evening urine samples for U-LH determinations over three consecutive days. No consistent day-to-day differences in U-LH levels were observed, although random variations, particularly in adolescents aged 13 or older, were noted. The net inter-assay CV% for U-LH changes over three days showed high variability, averaging 24.6% to 28.0% for boys and 21.6% to 27.3% for girls, independent of sex, collection time, or U-LH level. To reliably determine total urinary luteinizing hormone immunoreactivity in the pediatric population, it is advisable to collect multiple first-morning voided samples for at least three consecutive days as an interim solution, pending the development of a standardized protocol or correction method for varying urine composition. Strict adherence, especially for adolescents aged 13 or older, is vital.
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