Memory Recovery Effect of a New Bioactive Innovative Combination in Rats with Experimental Dementia
Lyubka Tancheva1, Reni Kalfin1,2, Borislav Minchev1
1Institute of Neurobiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. 23, 1113 Sofia, Bulgaria.
Abstract:
Alzheimer's disease manifests as a complex pathological condition, with neuroinflammation, oxidative stress and cholinergic dysfunction being a few of the many pathological changes. Due to the complexity of the disease, current therapeutic strategies aim at a multitargeted approach, often relying on a combination of substances with versatile and complementary effects. In the present study, a unique combination of α-lipoic acid, citicoline, extracts of leaves from olive tree and green tea, vitamin D3, selenium and an immune-supporting complex was tested in scopolamine-induced dementia in rats. Using behavioral and biochemical methods, we assessed the effects of the combination on learning and memory, and elucidated the mechanisms of these effects. Our results showed that, compared to its components, the experimental combination was most efficient in improving short- and long-term memory as assessed by the step-through method as well as spatial memory as assessed by T-maze and Barnes maze underlined by decreases in AChE activity (p < 0.05) and LPO (p < 0.001), increases in SOD activity in the cortex (p < 0.05) and increases in catalase (p < 0.05) and GPx (p < 0.01) activities and BDNF (p < 0.001) and pCREB (p < 0.05) levels in the hippocampus. No significant histopathological changes or blood parameter changes were detected, making the experimental combination an effective and safe candidate in a multitargeted treatment of AD.
Insights
A novel combination therapy significantly improved memory and cognitive function in rats with induced dementia. This multitargeted approach demonstrated effectiveness and safety for potential Alzheimer's disease treatment.
Area of Science:
- Neuroscience
- Pharmacology
- Biochemistry
Background:
- Alzheimer's disease (AD) involves complex pathologies like neuroinflammation and oxidative stress.
- Current AD treatments often require multitargeted strategies using combined substances.
Purpose of the Study:
- To evaluate a unique combination of supplements for treating scopolamine-induced dementia in rats.
- To assess the combination's effects on learning, memory, and underlying biochemical mechanisms.
Main Methods:
- Behavioral tests (step-through, T-maze, Barnes maze) assessed memory.
- Biochemical analyses measured acetylcholinesterase (AChE), lipid peroxidation (LPO), SOD, catalase, GPx, BDNF, and pCREB levels.
Main Results:
- The combination significantly improved short-term, long-term, and spatial memory compared to individual components.
- Key biochemical markers showed favorable changes: decreased AChE and LPO, increased SOD, catalase, GPx, BDNF, and pCREB.
- No adverse histopathological or blood parameter changes were observed.
Conclusions:
- The tested supplement combination is an effective and safe multitargeted strategy for managing dementia.
- This approach holds promise for future Alzheimer's disease therapeutic development.
