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SOX2-OT Binds with ILF3 to Promote Head and Neck Cancer Progression by Modulating Crosstalk between STAT3 and TGF-β
Ru Wang1,2, Yifan Yang1,2, Lingwa Wang1,2
1Department of Otorhinolaryngology, Head and Neck Surgery, Beijing Tong Ren Hospital, Capital Medical University, 1 Dongjiaominxiang Street, Beijing 100730, China.
Long non-coding RNA SOX2-OT promotes head and neck squamous cell carcinoma (HNSCC) progression and metastasis. Overexpression of SOX2-OT indicates a poor prognosis for HNSCC patients, suggesting it as a potential therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Long non-coding RNAs (lncRNAs) play a role in head and neck squamous cell carcinoma (HNSCC) development.
- The specific function of lncRNA SOX2-OT in HNSCC is not fully understood.
Purpose of the Study:
- To investigate the oncogenic role of lncRNA SOX2-OT in HNSCC.
- To explore the molecular mechanisms underlying SOX2-OT's function in HNSCC progression.
Main Methods:
- Quantitative reverse transcription PCR (QRT-PCR) and Fluorescence in situ hybridization (FISH) assays.
- In vitro and in vivo cell function assays, RNA pulldown, and RNA-binding protein immunoprecipitation (RIP) assays.
- Western blotting and Ingenuity pathway analysis.
Main Results:
- SOX2-OT was significantly overexpressed in HNSCC tissues and plasma, correlating with poor prognosis.
- SOX2-OT promoted HNSCC cell proliferation and metastasis.
- SOX2-OT interacts with ILF3 and regulates HNSCC progression via STAT3 phosphorylation and modulation of the STAT3/TGF-β signaling pathway.
Conclusions:
- SOX2-OT acts as an oncogene in HNSCC by interacting with ILF3.
- SOX2-OT may serve as a valuable prognostic biomarker and therapeutic target for HNSCC.
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