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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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Related Experiment Video

Updated: Jul 7, 2025

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs

Published on: June 12, 2018

Hepatitis B Virus and microRNAs: A Bioinformatics Approach.

Verdiana Zulian¹, Giulia Fiscon^2,3, Paola Paci^2,3

¹Virology Laboratory, National Institute for Infectious Diseases "Lazzaro Spallanzani" IRCCS, 00149 Rome, Italy.

International Journal of Molecular Sciences

|December 23, 2023

Summary

This summary is machine-generated.

Viral microRNAs (miRNAs) from hepatitis B virus (HBV) impact host gene expression, influencing disease progression. Bioinformatics tools are crucial for analyzing these complex viral-host interactions and developing diagnostic biomarkers.

Keywords:

antiviral treatment bioinformatics tools biomarkers chronic infection hepatitis B virus infectious diseases microRNA

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Last Updated: Jul 7, 2025

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Area of Science:

Molecular Biology
Virology
Bioinformatics

Background:

MicroRNAs (miRNAs) are key gene expression regulators.
Viral miRNAs (v-miRNAs), including those from hepatitis B virus (HBV), play significant roles in infection.
HBV infection can lead to chronic hepatitis B (CHB), fibrosis, cirrhosis, and hepatocellular carcinoma (HCC).

Purpose of the Study:

To review bioinformatics tools for viral and host miRNA research.
To provide an overview of miRNA roles in HBV infection.
To guide the selection of appropriate bioinformatics tools for researchers.

Main Methods:

Literature review of bioinformatics tools.
Analysis of existing research on miRNAs in HBV infection.
Synthesis of information on viral-host miRNA interactions.

Main Results:

miRNAs regulate host pathways like apoptosis and immune response during HBV infection.
v-miRNAs and host miRNAs are potential biomarkers for diagnosis and prognosis.
Bioinformatics is essential for analyzing complex miRNA data and viral-host interplay.

Conclusions:

Understanding miRNA dynamics in HBV infection is critical for clinical applications.
Bioinformatics tools facilitate the analysis and interpretation of miRNA data in viral infections.
This review aids researchers in selecting suitable bioinformatics tools for HBV-related miRNA studies.