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Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis In Vitro

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Published on: January 31, 2018

A Comprehensive Look at In Vitro Angiogenesis Image Analysis Software.

Mariana Pereira^1,2, Jéssica Pinto^1,2, Belén Arteaga^1,2,3

¹i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, 4200-135 Porto, Portugal.

International Journal of Molecular Sciences

|December 23, 2023

Summary

Developing vascularized tissue engineering (TE) constructs requires mimicking the extracellular matrix (ECM) to promote vessel growth. This review examines current standards, metrics, and software for evaluating microvessel development in TE.

Keywords:

angiogenesis biomaterials computational biomechanics image analysis tissue engineering

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Area of Science:

Biomaterials Science
Regenerative Medicine
Tissue Engineering

Background:

Vascularization is critical for tissue engineering (TE) success, enabling nutrient delivery and waste removal.
Current TE approaches face challenges in creating functional microvasculature that mimics native tissue extracellular matrix (ECM).

Purpose of the Study:

To review current standards and methodologies for evaluating microvessel development in TE constructs.
To survey available metrics and software for quantifying microvasculature and identify limitations.
To propose future improvements for angiogenesis evaluation in TE research.

Main Methods:

Literature review of tissue engineering techniques for vascularization.
Analysis of cell culture, scaffold selection, and biological cue requirements.
Survey of microscopy, labeling techniques, and quantification software for microvasculature analysis.

Main Results:

Standardized procedures for cell response analysis in TE are lacking.
Various metrics and software exist for microvessel quantification, but consistency is limited.
Current evaluation methods present analysis limitations impacting reproducibility.

Conclusions:

Standardization of evaluation protocols is essential for advancing TE vascularization.
Further development of robust metrics and software is needed for accurate angiogenesis assessment.
Addressing current limitations will accelerate the application of TE in wound healing and tissue regeneration.