Development and Application of an Automated Raman Sensor for Bioprocess Monitoring: From the Laboratory to an Algae...

Area of Science:

• Biotechnology
• Spectroscopy
• Microalgal Cultivation

Background:

• Microalgae are a source of valuable bio-components with economic and environmental significance.
• Current microalgal production monitoring relies on powerful but user-limited sensors and analytical methods.
• There is a need for automated, accessible monitoring solutions for microalgal cultivation.

Purpose of the Study:

• To develop and evaluate an automated Raman spectroscopy-based sensor for online monitoring of microalgal production.
• To enable real-time tracking of microalgal physiological states and intracellular molecules.
• To provide a more accessible monitoring tool for both laboratory and industrial settings.

Main Methods:

• An in situ automated system was designed, featuring a light-tight optical chamber connected to a Raman probe.
• Microalgal cultures were routed to the chamber via a computer-controlled system of pipes, pumps, and valves.
• Over 4000 Raman spectra were collected and analyzed using statistical methods from *Parachlorella kessleri* cultures.

Main Results:

• The generated Raman spectra effectively reflected the physiological state of the microalgal cells.
• The developed sensor demonstrated the capability to monitor intracellular molecules of interest.
• The system proved effective in complex laboratory and industrial algal production environments.

Conclusions:

• The automated Raman spectroscopy sensor offers a viable solution for online monitoring of microalgal production.
• This technology can provide valuable insights into microalgal physiology and biochemical composition in real-time.
• The developed system enhances accessibility and efficiency in monitoring microalgal cultivation.