Related Experiment Video

Updated: Jun 23, 2026

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
10:39

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

Published on: November 27, 2014

8.3K

PDIA4 Is a Host Factor Important for Lymphocytic Choriomeningitis Virus Infection

Mengwei Xu1, Huan Xu2, Weiwei Wan1

  • 1State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China.

Viruses
|December 23, 2023
PubMed

Insights

Researchers identified protein disulfide isomerase A4 (PDIA4) as crucial for lymphocytic choriomeningitis virus (LCMV) infection. Targeting PDIA4 offers a potential new strategy for developing antiviral therapies against LCMV.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Mammalian arenaviruses are zoonotic viruses causing severe hemorrhagic diseases.
  • Lymphocytic choriomeningitis virus (LCMV) is a significant arenavirus with no available FDA-approved treatments.
  • Host factors supporting LCMV replication remain incompletely understood.

Purpose of the Study:

  • To identify host factors essential for LCMV infection using quantitative proteomic analysis.
  • To investigate the role of protein disulfide isomerase A4 (PDIA4) in LCMV pathogenesis.
  • To explore potential therapeutic targets against LCMV.

Main Methods:

  • Quantitative proteomic analysis to identify host factors.
  • Biochemical assays to determine viral component interactions.
  • Endoplasmic reticulum stress (ERS) pathway manipulation (ATF6 inhibition).

Main Results:

  • PDIA4 was identified as a critical host factor for LCMV infection.
  • LCMV glycoprotein was found to upregulate PDIA4 expression.
  • Inhibiting ATF6-mediated ERS reduced LCMV-induced PDIA4 upregulation.
  • PDIA4 influences LCMV RNA synthesis and viral release.

Conclusions:

  • PDIA4 plays a significant role in the LCMV life cycle.
  • PDIA4 is a potential novel therapeutic target for LCMV antiviral drug development.
Keywords:
ATF6PDIA4arenaviruslymphoblastic choriomeningitis virus

More Related Videos

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease04:23

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

Published on: April 28, 2019

6.6K
Author Spotlight: Elucidating the Pathways of TFH Cell Differentiation in Acute LCMV Challenges05:03

Author Spotlight: Elucidating the Pathways of TFH Cell Differentiation in Acute LCMV Challenges

Published on: April 26, 2024

703

Related Concept Videos

Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Factors Affecting the Risk of Infection01:26

Factors Affecting the Risk of Infection

The hosts' susceptibility to infection depends on several factors. The integrity of the skin and mucous membranes helps protect the body against microbial attacks. When the skin is altered, the chance of infection, limb loss, and even death increases.
The integrity and count of the white blood cells help the body resist pathogens and fight infection. When impaired, it reduces the body's resistance to pathogens. The acidic pH levels of the gastrointestinal, genitourinary tracts, and skin create...
Immunodeficiency Diseases01:25

Immunodeficiency Diseases

Immunodeficiency disorders are conditions in which the immune system's ability to fight infectious disease and cancer is compromised or entirely absent. The immune system comprises a complex network of cells, tissues, and organs that work together to protect the body from potentially harmful invaders. When this system is deficient or not functioning properly, it leaves the body susceptible to infections, diseases, or other complications.
There are three main causes of immunodeficiency disorders...
Dipeptidyl Peptidase 4 Inhibitors01:23

Dipeptidyl Peptidase 4 Inhibitors

Dipeptidyl peptidase 4 (DPP-4) is a serine protease widely distributed in the body. It's involved in the inactivation of GLP-1 and GIP hormones, which are crucial for insulin regulation. DPP-4 inhibitors, such as sitagliptin (Januvia), saxagliptin (Onglyza), linagliptin (Tradjenta), alogliptin (Nesina), and vildagliptin (Galvus), help increase the proportion of active GLP-1, enhancing insulin secretion. These inhibitors work by competitively binding to DPP-4. This binding causes a significant...
Hepatitis01:25

Hepatitis

Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...
Gastritis II: Pathophysiology01:26

Gastritis II: Pathophysiology

The pathophysiology of gastritis begins with the colonization of the stomach lining by Helicobacter pylori (H. pylori). This bacterium spreads mainly via the oral-oral route through saliva or shared utensils, and can also be transmitted in overcrowded or unhygienic environments through contaminated water, despite its brief survival outside the body.ColonizationOnce ingested, H. pylori enters the stomach and begins colonization by navigating through the mucus layer lining the stomach wall. It...