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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Structural switching aptamer-based electrochemical sensor for mycotoxin patulin detection.

Netice Küçük1, Şevval Kaya2, Samet Şahin3

  • 1Bilecik Seyh Edebali University, Department of Biotechnology, Bilecik, Turkey.

Toxicon : Official Journal of the International Society on Toxinology
|December 23, 2023
PubMed
Summary

A new electrochemical aptasensor detects the mycotoxin patulin in fruit products. This sensitive sensor uses a structural switching mechanism for reliable food safety analysis.

Keywords:
Apple-juiceAptasensorPatulinSquare wave voltammetryStructural switching

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Area of Science:

  • Electrochemistry
  • Biosensors
  • Food Science

Background:

  • Patulin is a harmful mycotoxin frequently contaminating fruits and fruit products.
  • Accurate detection methods are crucial for ensuring food safety and quality control.
  • Electrochemical aptasensors offer a promising platform for sensitive mycotoxin detection.

Purpose of the Study:

  • To develop a novel electrochemical aptasensor for sensitive patulin detection.
  • To utilize an innovative structural switching signal-off mechanism for patulin quantification.
  • To evaluate the aptasensor's performance in real food samples.

Main Methods:

  • Fabrication of aptasensor using screen-printed carbon electrodes with Au electrodeposition and thiol chemistry.
  • Optimization of incubation times using response surface methodology.
  • Detection of patulin via square wave voltammetry based on structural switching.

Main Results:

  • The aptasensor demonstrated a linear working range of 1-25 μM and a low detection limit of 3.56 ng/mL for patulin.
  • High accuracy (94.4%) and low relative standard deviation (0.067) were achieved.
  • The sensor showed good stability over 10 days and minimal interference from other mycotoxins at higher concentrations.

Conclusions:

  • The developed electrochemical aptasensor is effective for sensitive patulin detection in fruit-based products.
  • The structural switching signal-off platform provides a reliable detection strategy.
  • This aptasensor holds significant potential for applications in food safety and quality control.