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Updated: Aug 5, 2026
A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants
Published on: February 19, 2016
Construction of SERS output-signal aptasensor using MOF/noble metal nanoparticles based nanozyme for sensitive
Xiaoyuan Ma1, Shan Xu1, Yue Pan1
1State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi 214122, China.
Abstract:
Herein, a signal output SERS aptasensor for Histamine (HA) detection is designed. MIL-100(Fe) was loaded with gold nanoparticles (AuNPs) to form composite nanozyme MIL-100(Fe)@AuNPs, which was used in the reaction system TMB/H2O2. Silver nanoparticles (AgNPs) were synthesized as "amplifier" for the SERS signal of ox TMB. After nucleic acid functionalization, the two parts were assembled to form the multifunctional substrate with both high catalytic and SERS efficiency. In the detection system, the specific binding effect of HA aptamer toward HA induced a decrease in the assembly of AgNPs on MIL-100(Fe)@AuNPs which caused a decrease in ox TMB SERS signals. The linear relation of HA ranged from 10-11 M to 5 × 10-3 M with LOD as low as 3.9 × 10-12 M. Recovery ratio in fermented soybean products (94.42-105.75 %) proved the real sample applicability. The fabricated SERS aptasensor will provide technical support for the safety during food processing and storage.
