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Related Experiment Video

Updated: Jul 7, 2025

Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel
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Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel

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Encapsulated human islets in alginate fiber maintain long-term functionality.

Junko Nishida1, Takahiro Tsuno2, Shigeharu G Yabe1

  • 1Department of Regenerative Medicine, Research Institute, National Center for Global Health and Medicine, Tokyo 162-8655, Japan.

Endocrine Journal
|December 24, 2023
PubMed
Summary

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This summary is machine-generated.

Alginate encapsulation maintains human islet function and glucose responsiveness for over 180 days in vitro. This method preserves islet viability and secretion, crucial for transplantation and research.

Area of Science:

  • Endocrinology
  • Biomaterials Science
  • Transplantation Biology

Background:

  • Maintaining islet function during in vitro culture is critical for islet transplantation and research.
  • Human islets are susceptible to functional decline when cultured ex vivo.

Purpose of the Study:

  • To evaluate the impact of alginate fiber encapsulation on the long-term function and viability of human islets cultured in vitro.
  • To assess the glucose responsiveness of encapsulated human islets over an extended culture period.

Main Methods:

  • Human islets from 15 deceased donors were encapsulated in alginate fibers.
  • Encapsulated islets were cultured in vitro at 5.5 mM or 25 mM glucose.
  • C-peptide and glucagon secretion were measured periodically.
Keywords:
AlginateHuman isletLong-term culture

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  • Immunohistochemical studies assessed islet composition and beta-cell survival.

    • Main Results:

    • Encapsulated islets maintained C-peptide and glucagon secretion for over 75 days in 5 cases, with detection up to 180 days in 2 cases.
    • Alpha- and beta-cell composition and beta-cell survival remained unaltered after 75 or 180 days of culture.
    • Islets cultured at 5.5 mM glucose retained glucose responsiveness of C-peptide secretion until day 180, unlike those at 25 mM glucose.

    Conclusions:

    • Alginate encapsulation significantly enhances the long-term viability and functional maintenance of human islets in vitro.
    • Encapsulation preserves the glucose-responsive C-peptide secretion of human islets for potentially over 180 days.
    • This technique offers a promising strategy for improving islet preservation for transplantation and research applications.