Preparation of surface plasmon resonance-based nanosensor for curcumin detection

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Materials Science

Background:

• Curcumin detection is crucial for pharmaceutical and food industries.
• Developing selective and sensitive detection methods remains a challenge.
• Nanoparticle-based SPR nanosensors offer promising solutions for analyte detection.

Purpose of the Study:

• To prepare and characterize curcumin imprinted and non-imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-tryptophan) (poly(HEMA-MATrp)) nanoparticle-based SPR nanosensors.
• To evaluate the performance of these nanosensors for curcumin detection.
• To validate the nanosensors' performance in real samples.

Main Methods:

• Preparation and characterization of poly(HEMA-MATrp) nanoparticles using zeta-size analysis, FTIR, and SEM.
• Immobilization of nanoparticles onto SPR chips and characterization using AFM, ellipsometry, and contact angle measurements.
• Kinetic studies and performance evaluation (LOD, LOQ) of SPR nanosensors for curcumin detection.
• Validation using liquid chromatography-tandem mass spectrometry (LC-MS).

Main Results:

• Successfully prepared and characterized curcumin imprinted and non-imprinted nanoparticles and SPR nanosensors.
• Achieved a rapid response time of 14 min for kinetic studies.
• Demonstrated a low limit of detection (0.0012 mg/L) and limit of quantification (0.0040 mg/L) for curcumin using imprinted SPR nanosensors.
• Validated the nanosensor's effectiveness in real samples.

Conclusions:

• Curcumin imprinted poly(HEMA-MATrp) nanoparticle-based SPR nanosensors are effective for sensitive and selective curcumin detection.
• The developed nanosensors show potential for practical applications in curcumin analysis.
• This approach offers a valuable tool for quality control and research involving curcumin.