Novel poly (N-methacryloyl-L-alanine acid) grafted chitosan microspheres based solid-phase extraction coupled with...

Poly (N-methacryloyl-L-alanine acid) grafted tartaric acid-crosslinked chitosan microspheres (PNMA-TACS) were successfully synthesized and employed as a novel adsorbent for the separation and enrichment of metal ions in the food system. PNMA-TACS microspheres-based solid phase extraction (SPE) was coupled with ICP-MS for accurate quantification of trace V(V), Cr(III), As(III), Pb(II), Cd(II) and Cu(II). The obtained PNMA-TACS microspheres were characterized, and parameters influencing the method were optimized. Under optimal conditions, the calibration curves for Cu(II) and V(V) were linear within 0.01-30 μg L^-1, the linear ranges of Cr(III), As(III), Pb(II) and Cd(II) were 0.01-15 μg L^-1, and the detection limit of the developed approach was 1.1-3.7 ng L^-1. The results were consistent with the consensus values of method validation implemented by two standards. Moreover, standard addition recovery experiments were performed in rice and milk powder, which achieved satisfactory recovery of 86.1-103.5%.