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Reference intervals for serum immunoglobulins G, M and E levels in Brazilian children aged 1 to 10 years: a
Sandra Breder Assis1, Natasha Slhessarenko1, Cor Jesus Fernandes Fontes2
1Universidade Federal de Mato Grosso (UFMT), Faculdade de Medicina, Departamento de Pediatria, Cuiabá, MT, Brazil.
Insights
Reference intervals for immunoglobulin G (IgG), immunoglobulin M (IgM), and immunoglobulin E (IgE) serum levels were established for healthy Brazilian children aged 1-10 years. These findings provide crucial data for clinical decision-making in pediatric healthcare.
Area of Science:
- Pediatric Immunology
- Clinical Chemistry
- Reference Interval Studies
Background:
- Accurate reference intervals (RI) for serum immunoglobulin levels are essential for diagnosing and managing various pediatric conditions.
- Existing RI may not accurately reflect the diverse pediatric population in central Brazil.
- This study addresses the need for region-specific immunoglobulin RI in children.
Purpose of the Study:
- To determine the reference intervals (RI) for serum immunoglobulin G (IgG), immunoglobulin M (IgM), and immunoglobulin E (IgE) in healthy children aged 1-10 years.
- To provide updated and relevant laboratory parameters for Brazilian pediatricians.
- To facilitate accurate clinical decision-making in pediatric immunology.
Main Methods:
- A cross-sectional study involving 1743 healthy children aged 1-10 years from central Brazil.
- Serum samples were analyzed for IgG, IgM, and IgE levels.
- Reference intervals were established using non-parametric rank, bootstrapping, and Horn's robust methods.
Main Results:
- The IgG RI for children aged 1-10 years was 792-1771 mg/dL.
- IgM RI varied by gender and age group, with specific ranges provided for boys and girls across different age brackets.
- The IgE RI for both sexes aged 1-10 years was 6-1005 mg/dL.
- Bootstrap and Horn's methods yielded comparable yet distinct RI, highlighting the importance of methodological choice.
Conclusions:
- Established reference intervals for serum IgG, IgM, and IgE in Brazilian children aged 1-10 years.
- These region-specific RI will aid Brazilian physicians in making more informed clinical decisions.
- The study contributes valuable data for pediatric diagnostic and monitoring protocols in Brazil.
Objective:
To determine the reference intervals (RI) for serum levels of IgG, IgM, and IgE in healthy children aged 1-10 years living in central Brazil.
Method:
A sample of 1743 healthy children was randomly selected from kindergartens and public schools. Reference intervals were defined by non-parametric rank (Clinical Laboratory Standards Institute, USA), bootstrapping, and Horn's robust methods.
Results:
By the rank method, the IgG RI was 792-1771 mg/dL for children of both sexes aged 1-10 years. IgM RI were different for gender and age groups, being 45-196 mg/dL and 34-190 mg/dL for boys aged 1-2 years and 3-10 years, respectively. For girls, the IgM RI were 50-212 mg/dL and 39-212 mg/dL, for ages 1-4 and 5-10 years, respectively. The IgE RI for both sexes and ages 1-10 years was 6-1005 mg/dL. The bootstrap method showed RI similar to the rank method but with slightly different confidence intervals. Horn's robust method determined RI different from those obtained by previous methods.
Conclusion:
RI for serum concentrations of IgG, IgM, and IgE were established for Brazilian children aged 1-10 years. This definition will be useful for Brazilian physicians, who will have more adequate parameters for their clinical decision-making.
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