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lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

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Updated: Jul 7, 2025

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

Techniques for investigating lncRNA transcript functions in neurodevelopment.

Tara Srinivas¹, Edilene Siqueira¹, Sonia Guil^2,3

¹Josep Carreras Leukaemia Research Institute (IJC), 08916, Badalona, Barcelona, Catalonia, Spain.

Molecular Psychiatry

|December 25, 2023

Summary

This summary is machine-generated.

Investigating long noncoding RNAs (lncRNAs) in the central nervous system (CNS) is challenging due to their complex nature. This review details methods for studying lncRNA function in the CNS, aiding neurodevelopment and pathophysiology research.

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Area of Science:

Genomics
Neuroscience
Molecular Biology

Background:

Long noncoding RNAs (lncRNAs) are transcribed from a significant portion of the mammalian genome.
Many lncRNAs have uncharacterized functions due to challenges like genomic overlap and cell-type-specific expression.
lncRNAs are abundant and diverse within the mammalian central nervous system (CNS).

Purpose of the Study:

To review current methods for investigating lncRNA function in the CNS.
To discuss the advantages and limitations of these methods.
To provide recommendations for assaying lncRNAs in CNS research.

Main Methods:

Review of existing literature on lncRNA investigation techniques.
Analysis of methods applicable to CNS models, including in vivo studies.
Discussion of challenges specific to studying lncRNAs in the brain.

Main Results:

A comprehensive overview of diverse methods for lncRNA research in the CNS.
Evaluation of how different methods contribute to understanding neurodevelopment and pathophysiology.
Identification of persistent challenges in the field.

Conclusions:

Effective investigation of lncRNAs in the CNS requires careful selection of appropriate methods.
Further development of techniques is needed to overcome current limitations.
Standardized approaches can advance the understanding of lncRNA roles in neurological processes.