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Lipidomics and Transcriptomics in Neurological Diseases
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Datasets assessing lipid-content in optically cleared brains.

Shimrit Oz1, Galit Saar2, Shunit Olszakier1

  • 1Department of Neuroscience, Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.

Data in Brief
|December 26, 2023
PubMed
Summary
This summary is machine-generated.

Tissue clearing methods impact lipid content and Magnetic Resonance Imaging (MRI) contrast in cleared brains. Delipidation significantly reduces MRI signal, with varying effects depending on the clearing technique used.

Keywords:
Fluorescent probeHistochemistryImagingLipidsMRITissue-clearing

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Area of Science:

  • Neuroscience
  • Biomedical Imaging
  • Histology

Background:

  • Multi-modal imaging combines light microscopy (LM) and Magnetic Resonance Imaging (MRI) for brain examination at various scales.
  • Tissue clearing renders whole brains transparent for LM, requiring lipid removal (delipidation).
  • The impact of delipidation on MRI contrast in cleared tissues remains debated.

Purpose of the Study:

  • To investigate the relationship between lipid content, tissue clearing techniques, and MRI contrast.
  • To assess the effects of different clearing methods on lipid preservation and tissue integrity.

Main Methods:

  • Mouse and rat brains were cleared using CLARITY, Scale, uDISCO, and ECi techniques.
  • Lipid content was assessed using Oil Red O (ORO) and DiI staining, and MRI contrast-to-noise ratio was measured.
  • Tissue integrity and dye washout were analyzed at various stages of clearing.

Main Results:

  • CLARITY's lipid electrophoresis reduced MRI contrast in both control and cleared brains.
  • ORO staining was minimal in CLARITY-cleared brains but efficient in uDISCO and ECi-cleared brains.
  • DiI staining showed consistent results across all clearing methods, while CLARITY impaired tissue integrity more than uDISCO and ECi.

Conclusions:

  • Different tissue clearing methods exhibit variable effects on lipid content and MRI contrast.
  • Lipid removal during clearing significantly impacts MRI signal, with technique-dependent outcomes.
  • These findings aid in understanding lipid contributions to brain MRI contrast and optimizing clearing protocols.