GC-MS based metabolic profile and toxicological evaluation of three Algerian propolis

This study aimed to investigate the chemical composition and toxicity of methanolic extracts of three Algerian propolis collected from Oum el Bouaghi (MEPO), El Harrouch (MEPH) and Collo (MEPC) regions. The chemical profile was characterised by GC-MS. The toxicity of the extracts was tested using brine shrimp model. The GC-MS analysis revealed the presence of ferulic acid (23.8%), pinostrobin chalcone (15.8%) and α-eudesmol (11.3%) as major compounds in MEPO, pinostrobin chalcone (22.2%), 9-octadecenoic acid, methyl ester, (E)- (17.4%), and γ-gurjunenepoxide-(2) (11.7%) were the most abundant components in MEPH, whereas MEPC was dominated by 1-heptatriacotanol (17.8%), pinostrobin chalcone (14.7%), totarol (13.7%) and 9-octadecenoic acid, methyl ester, (E)- (13.0%). The brine shrimp lethality test indicated that the extracts had moderate toxicity in which MEPC exhibited the highest activity with LC₅₀ of 201.61 ± 7.27 µg/mL. All extracts showed no toxicity at 25 µg/mL concentration and below.