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Sweet cherry AP2/ERF transcription factor, PavRAV2, negatively modulates fruit size by directly repressing PavKLUH
Xiliang Qi1,2, Lifeng Liu1,2, Congli Liu1,2
1Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, China.
Physiologia Plantarum
|December 26, 2023
Summary
Researchers identified a key gene, PavRAV2, that regulates sweet cherry fruit size by controlling the expression of PavKLUH. This discovery offers insights into plant breeding for larger fruits.
Area of Science:
- Plant biology
- Genetics
- Agricultural science
Background:
- Fruit size is crucial for sweet cherry breeding due to market demand.
- The KLUH/CYP78A5 gene regulates seed and fruit size in plants, but its mechanism is not fully understood.
Purpose of the Study:
- To investigate the role of PavKLUH in sweet cherry fruit size regulation.
- To identify molecular mechanisms controlling fruit size in sweet cherries.
Main Methods:
- Ectopic overexpression of PavKLUH in Arabidopsis.
- Virus-induced gene silencing of PavKLUH in sweet cherry.
- Yeast one-hybrid (Y1H) assays, electrophoretic mobility shift assays, and dual-luciferase reporter analyses to study PavRAV2 and PavKLUH interaction.
Main Results:
- Overexpressing PavKLUH increased Arabidopsis silique and seed size.
- Silencing PavKLUH in sweet cherry reduced fruit size by limiting mesocarp cell expansion.
- PavRAV2, an AP2/ERF transcription factor, directly binds to and suppresses PavKLUH promoter activity.
- Silencing PavRAV2 led to larger sweet cherry fruits due to increased mesocarp cell expansion.
Conclusions:
- PavRAV2 directly regulates PavKLUH expression, influencing sweet cherry fruit size.
- This study elucidates a novel signaling pathway involving RAV transcription factors and CYP78A family members in fruit development.
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