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Intestinal Trefoil Factor 3: a new biological factor mediating gut-kidney crosstalk in diabetic kidney disease
Tao Zhang1, Yinghui Zhang1, Jie Tao1
1Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine, Key Laboratory of Glucolipid Metabolic Disorder, Ministry of Education; Guangdong TCM Key Laboratory for Metabolic Diseases, Guangzhou Higher Education Mega Center, Institute of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China.
Purpose:
To investigate the effect of TFF3 in the pathogenesis of Diabetic Kidney Disease (DKD), and explore the dynamic changes of TFF3 expression pattern in renal injury process.
Methods:
DKD animal model was established by streptozotocin (STZ) (40 mg/kg/d, ip, for 5 days, consecutively) combined with the high fat diet (HFD) for 12 weeks. While animals were sacrificed at different time stages in DKD process (4 weeks, 8 weeks and 12 weeks, respectively).
Results:
STZ combined with high-fat diet induced weight gain, increased blood glucose and decreased glucose tolerance in DKD mice. Compared to the control group, the DKD group exhibits extracellular matrix (ECM) accumulation and the renal injury was aggravated in a time-dependent manner. The TFF3 expression level was decreased in kidney, and increased in colon tissue.
Conclusion:
TFF3 is not only expressed in colon, but also expressed in renal medulla and cortex. TFF3 might be play a pivotal role in renal mucosal repair by gut-kidney crosstalk, and protect renal from high glucose microenvironment damage.
Insights
Trefoil Factor 3 (TFF3) expression decreases in the kidneys during diabetic kidney disease (DKD) progression. TFF3 may protect the kidneys via gut-kidney communication, mitigating high glucose damage.
Area of Science:
- Nephrology
- Endocrinology
- Gastroenterology
Background:
- Diabetic Kidney Disease (DKD) is a major complication of diabetes.
- Understanding the molecular mechanisms underlying DKD pathogenesis is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the role of Trefoil Factor 3 (TFF3) in DKD pathogenesis.
- To analyze the dynamic changes in TFF3 expression during renal injury in DKD.
Main Methods:
- A diabetic kidney disease (DKD) mouse model was established using streptozotocin (STZ) and a high-fat diet (HFD).
- Animals were analyzed at different time points (4, 8, and 12 weeks) to assess disease progression.
- TFF3 expression levels were measured in kidney and colon tissues.
Main Results:
- The DKD model exhibited hyperglycemia, impaired glucose tolerance, and progressive renal injury with extracellular matrix accumulation.
- TFF3 expression was significantly decreased in kidney tissues over time.
- Conversely, TFF3 expression increased in colon tissues of DKD mice.
Conclusions:
- TFF3 is expressed in both the colon and renal medulla/cortex.
- TFF3 may play a protective role in renal mucosal repair through gut-kidney crosstalk.
- TFF3 could mitigate kidney damage caused by a high-glucose environment in DKD.
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