Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Expansion Revealing of Pathology Resolves Nanostructures Associated with Inflammatory Phenotypes in COVID-19 Decedent Human Brain Tissue.

bioRxiv : the preprint server for biology·2026

Same author

Humanized extracellular vesicles for efficient RNA delivery.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Massively parallel reporter assay for mapping gene-specific regulatory regions at single-nucleotide resolution.

eLife·2026

Same author

Scaling Up Sequential Stepwise DNA Displacement-Based Signal Exchange for Rapid Ultraplex Fluorescent Imaging in Cells and Tissues.

Journal of the American Chemical Society·2026

Same author

Programmable fluorescent aptamer-based RNA switches for rapid identification of point mutations.

Nature chemistry·2025

Same author

Author Correction: Arc in the nucleus regulates PML-dependent GluA1 transcription and homeostatic plasticity.

Nature neuroscience·2025

Search research articles

Related Experiment Video

Updated: Jul 7, 2025

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Thermal-plex: fluidic-free, rapid sequential multiplexed imaging with DNA-encoded thermal channels.

Fan Hong^1,2,3, Jocelyn Y Kishi^1,2, Ryan N Delgado⁴

¹Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.

|December 27, 2023

Summary

Thermal-plex enables rapid, fluidic-free multiplexed fluorescence imaging by using DNA probes activated by heat. This method achieves 15-plex RNA imaging in under 4 minutes, overcoming limitations of traditional sequential imaging.

More Related Videos

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

Published on: March 30, 2020

Related Experiment Videos

Last Updated: Jul 7, 2025

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

Published on: March 30, 2020

Area of Science:

Biotechnology
Molecular Biology
Microscopy

Background:

Multiplexed fluorescence imaging is crucial for biological research but often limited by the number of detectable targets.
Existing sequential imaging methods require complex fluidics and lengthy buffer exchange steps, hindering speed and accessibility.

Purpose of the Study:

To introduce Thermal-plex, a novel fluidic-free method for rapid, high-plex sequential fluorescence imaging.
To overcome the limitations of conventional sequential imaging techniques.

Main Methods:

Developed simple DNA probes activated by specific temperature spikes (thermal channels) for sequential fluorescence activation.
Utilized a commercially available heating device for rapid channel switching (<30 seconds).
Performed imaging without buffer exchange or complex fluidic systems.

Main Results:

Demonstrated 15-plex RNA imaging (5 thermal channels × 3 fluorescence channels) in fixed cells and retina tissues.
Achieved complete 15-plex imaging in under 4 minutes.
Showcased the speed and efficiency of the fluidic-free Thermal-plex approach.

Conclusions:

Thermal-plex offers a significantly faster and simpler alternative for high-multiplexed sequential imaging.
This method expands the capabilities of fluorescence microscopy for biological research.
Introduced a new labeling strategy for efficient sequential multiplexed imaging.