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Updated: Jul 7, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Gut microbiome composition: link between sports performance and protein absorption?
Péter Fritz1, Réka Fritz2, Pál Bóday3
1Károli Gáspár University of the Reformed Church in Hungary, Faculty of Economics, Health Sciences and Social Studies, Budapest, Hungary.
Elite athletes consuming plant-based protein with pre- and probiotics showed improved body composition. Gut microbiota modulation positively influenced skeletal muscle mass and body fat, enhancing athletic potential.
Area of Science:
- Sports Science
- Microbiology
- Nutritional Science
Background:
- Adequate protein intake is crucial for athletic performance.
- Protein absorption is influenced by factors like timing, type, and gut microbiota.
- Elite male water polo players were studied to assess protein supplement effects.
Purpose of the Study:
- To investigate the impact of a vegan protein supplement with pre- and probiotics on elite water polo players.
- To analyze changes in body composition, gut microbiota, and blood parameters.
- To explore the correlation between gut microbiota composition and physiological changes.
Main Methods:
- Participants received a vegan protein supplement with or without pre- and probiotics for 31 days.
- Body composition was measured using bioimpedance analysis (InBody 970).
- Gut microbiota composition was analyzed via metagenome sequencing of stool samples.
Main Results:
- The group consuming pre- and probiotics showed significant increases in skeletal muscle mass, body cell mass, arm circumference, and protein mass.
- This group also experienced a significant decrease in body fat mass.
- Specific gut microbial pathways (acetate and propionate) correlated with muscle mass, and butyrate producers correlated with fat mass changes.
Conclusions:
- Gut microbiota composition can influence protein absorption, impacting body composition and athletic performance.
- Supplementation with pre- and probiotics alongside vegan protein may enhance body composition in athletes.
- Modulating the gut microbiome presents a potential strategy for optimizing athletic outcomes.
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