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Split-GFP lamin as a tool for studying C. elegans LMN-1 dynamics in vivo
Ellen F Gregory1, James Matthew Ragle2, Jordan D Ward2
1Department of Molecular and Cellular Biology, University of California, Davis, Davis, California, United States.
Abstract:
We engineered a fluorescent fusion protein of C. elegans lamin, by fusing the eleventh beta strand of GFP to the N-terminus of LMN-1 at the endogenous lmn-1 locus. When co-expressed with GFP1-10, GFP11::LMN-1 was observed at the nuclear periphery of a wide variety of somatic cells. Homozygous gfp11::lmn-1 animals had normal numbers of viable embryos. However, the gfp11::lmn-1 animals had a mild swimming defect. While not completely functional, the GFP11::LMN-1 strain is more healthy than other published fluorescent LMN-1 lines, making it a valuable reagent for studying lamins.
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