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Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
Published on: October 17, 2017
Blood Monocyte Phenotype Is A Marker of Cardiovascular Risk in Type 2 Diabetes
Jean-Baptiste Julla1,2,3,4, Diane Girard1,2,3, Marc Diedisheim1,2,3,5
1INSERM, Necker Enfants Malades (INEM), INSERM U1151, CNRS UMR 8253, IMMEDIAB Laboratory (J.-B.J., D.G., M.D., B.T.V., C.B., E.C., L.O., I.N., C.P., K.K., D.C.T., T.E., R.B., J.-P.R., L.P., R.R., C.V., F.A., J.-F.G., N.V.), Université Paris Cité, France.
High monocyte counts in type 2 diabetes (T2D) indicate increased atherosclerotic cardiovascular disease risk. Monocyte profiling offers a valuable predictive marker for cardiovascular events in T2D patients.
Area of Science:
- Cardiovascular Research
- Immunology
- Metabolomics
Background:
- Diabetes significantly elevates atherosclerotic cardiovascular disease (ASCVD) risk.
- Circulating monocytes are key inflammatory cells implicated in type 2 diabetes (T2D) and atherogenesis.
Purpose of the Study:
- To investigate the link between circulating monocytes and cardiovascular risk progression in T2D patients.
- To identify monocyte-based biomarkers for predicting cardiovascular events in T2D.
Main Methods:
- Phenotypic, transcriptomic, and metabolomic analyses of circulating monocytes.
- Coronary artery calcium (CAC) scoring to assess cardiovascular risk in 672 T2D patients.
- Validation in independent cohorts totaling 1036 T2D participants.
Main Results:
- Monocyte count and classical monocyte frequency correlated positively with CAC score.
- Three distinct T2D endotypes based on monocyte profiles were identified, correlating with varying cardiovascular event risk.
- Disrupted mitochondrial pathways in monocytes indicated a proatherogenic phenotype.
Conclusions:
- Monocyte frequency and phenotype are closely associated with cardiovascular risk in T2D.
- Monocyte count serves as a valuable predictive marker for cardiovascular events in T2D patients.
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