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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
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Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

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MicroRNA profiling in complete and partial hydatidiform moles.

W K Chia1, H X Y Yeoh1, M Mustangin1

  • 1Universiti Kebangsaan Malaysia, Faculty of Medicine, Department of Pathology, Jalan Yaacob Latif, Bandar Tun Razak, Kuala Lumpur, Malaysia.

The Malaysian Journal of Pathology
|December 28, 2023
PubMed
Summary
This summary is machine-generated.

MicroRNAs (miRNAs) were identified as differentially expressed in complete (CM) and partial moles (PM). miR-518 was notably downregulated in CM, suggesting its potential role as a biomarker for hydatidiform mole progression.

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Area of Science:

  • Reproductive biology
  • Molecular genetics
  • Oncology

Background:

  • Hydatidiform mole, a gestational trophoblastic disease, includes complete (CM) and partial moles (PM).
  • These conditions carry risks of developing persistent disease, invasive mole, or choriocarcinoma.
  • The role of microRNAs (miRNAs) in molar pregnancy pathogenesis and as biomarkers remains largely uncertain.

Purpose of the Study:

  • To identify differentially expressed miRNAs in complete moles (CM) and partial moles (PM).
  • To investigate the potential role of these miRNAs in the pathogenesis of hydatidiform moles (HMs).
  • To explore the utility of miRNAs as diagnostic biomarkers and therapeutic targets for HMs.

Main Methods:

  • Next-generation sequencing was employed to profile miRNAs in CM (n=3), PM (n=3), and non-molar abortus placentas (n=3) as controls.
  • Differential expression analysis was performed between the groups.
  • Bioinformatics tools (miRDB, Targetscan) were used to predict target genes of differentially expressed miRNAs.

Main Results:

  • Ten differentially expressed miRNAs were identified between CMs, PMs, and normal molar tissues (NMAs).
  • Specific miRNAs, including miR-518a-5p, miR-423-3p, miR-503-5p, miR-302a-3p, and miR-1323, were identified.
  • Three differentially expressed miRNAs in CMs were predicted to target ZTBT46 and FAM73B mRNAs.

Conclusions:

  • miR-518 was consistently downregulated in CM compared to both PM and NMA.
  • Further bioinformatic analysis is required to elucidate the role of these miRNAs in HM pathogenesis and disease progression.
  • These miRNAs show potential as diagnostic biomarkers and therapeutic targets for hydatidiform moles.