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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
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Ribosomal protein L8 regulates the expression and splicing pattern of genes associated with cancer-related pathways

Leilei Xu1, Gui Yang1, Bin Song2

  • 1Department of Bone Tumor, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, P.R. China.

Turkish Journal of Biology = Turk Biyoloji Dergisi
|December 29, 2023
PubMed
Abstract

Insights

Ribosomal protein L8 (RPL8) overexpression inhibits cancer cell proliferation and promotes apoptosis by altering gene expression and alternative splicing. This study reveals novel insights into RPL8's role in tumor development.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genomics

Background:

  • Ribosomal proteins have extraribosomal functions, including roles in apoptosis and oncogenesis.
  • Ribosomal protein L8 (RPL8) is implicated in tumor development, but its specific functions and pathways remain unclear.

Purpose of the Study:

  • To investigate the biological functions and pathways of RPL8 in cancer.
  • To analyze the effects of RPL8 overexpression on cell proliferation, apoptosis, gene expression, and alternative splicing.

Main Methods:

  • Overexpression of RPL8 (RPL8-OE) in HeLa cells.
  • MTT assay for cell proliferation and flow cytometry for apoptosis.
  • Transcriptome sequencing to identify differentially expressed genes (DEGs) and regulated alternative splicing events (RASEs), validated by RT-qPCR.

Main Results:

  • RPL8-OE inhibited cell proliferation and promoted apoptosis.
  • RPL8 regulated DEGs and RASEs involved in tumorigenesis, angiogenesis, inflammation, and cell proliferation.
  • RPL8 influenced genes related to apoptosis regulation and potentially modulated transcription factors via alternative splicing.

Conclusions:

  • RPL8 affects cancer cell phenotypes by altering transcriptome profiles, including gene expression and splicing.
  • These findings offer new perspectives on the biological functions of RPL8 in tumor development.
Keywords:
DEGsRPL8alternative splicingcancertranscription factors

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