Pyrene-Based "Turn-On" Fluorescent Polymeric Probe with Thioacetal Units in the Main Chain for Mercury(II) Detection...

A water-soluble polymeric pyrene-based polythioacetal (PTA-Py) with thioacetal units in the main chain is simply synthesized by direct polycondensation of 3, 6-dioxa-1, 8-octanedithiol, 1-pyrene formaldehyde, and mPEG2k-SH. The probe PTA-Py shows a good fluorescence response to Hg²⁺ ions due to the Hg²⁺-promoted deprotection reaction of thioacetal groups to regenerate the original 1-pyrene formaldehyde compound. After adding Hg²⁺ to the PTA-Py solution, the fluorescence intensity (FI) gradually increases with increasing concentrations of Hg²⁺. Compared with other metal ions, the probe exhibits high sensitivity, good selectivity, and rapid response to Hg²⁺. The low detection limits are 12.3 nm in ethanol-PBS buffer and 13.3 nm in water, respectively. The results imply that the simply synthesized water-soluble polymeric probe had potential applications in the rapid detection of Hg²⁺ ions in aqueous solutions. Moreover, the polymeric PTA-Py shows high sensitivity for CH₃Hg⁺ with detection limits of 26.5 nm in ethanol/PBS buffer. In addition, PTA-Py can efficiently detect Hg²⁺ ions in HeLa cells. The results demonstrate that a valuable method is developed for biocompatible polymeric sensors for Hg²⁺ ions in biological and environmental samples.