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Related Experiment Videos

Functional C8 associated with human platelets.

F Tedesco, P Densen, M A Villa

    Clinical and Experimental Immunology
    |November 1, 1986
    PubMed
    Summary
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    Complement component 8 (C8) is functionally active on human platelets, playing a role in their hemolytic activity. Platelet-bound C8 is distinct from the C5b-9 complex and is not released during platelet activation.

    Area of Science:

    • Immunology
    • Hematology
    • Complement System

    Background:

    • The complement system is crucial for innate and adaptive immunity.
    • Complement component 8 (C8) is a key protein in the terminal pathway of complement activation.
    • The specific role and localization of C8 within human platelets remain incompletely understood.

    Purpose of the Study:

    • To investigate the presence and functional activity of C8 on human platelets.
    • To determine the association of C8 with platelets and its potential role in platelet-mediated functions.
    • To differentiate platelet-associated C8 from the membrane attack complex (C5b-9).

    Main Methods:

    • Haemolytic assays were performed using washed human platelets.
    • Inhibition studies utilized F(ab')2 anti-C8 and F(ab')2 anti-C5 antibodies.

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  • Platelets from C8-deficient patients were used to assess C8 dependency.
  • Biochemical analysis included platelet homogenization, centrifugation, and Sephacryl S-200 gel filtration.

    • Main Results:

    • Haemolytic assays confirmed the presence of functionally active C8 on human platelets.
    • Platelet-bound C8 activity was inhibited by anti-C8 antibodies and absent in C8-deficient individuals.
    • Platelets from C8-deficient patients did not acquire C8 activity when incubated in normal plasma.
    • C8 was detected as an individual component, not part of the C5b-9 complex, and was not released upon platelet activation by thrombin.

    Conclusions:

    • Human platelets possess functionally active C8 as an intrinsic component.
    • Platelet-associated C8 is independent of plasma C8 and is not released during platelet activation.
    • This finding suggests a potential novel role for C8 in platelet biology beyond its canonical function in complement-mediated cell lysis.