Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Rolling With Slipping01:14

Rolling With Slipping

4.9K
Rolling with slipping is a physical phenomenon that occurs when a rolling object experiences both rotational and linear motion but also experiences frictional forces that cause slipping. This phenomenon can occur in various situations, such as when a tire rolls on a wet road or a ball rolls on a rough surface.
An object's rolling motion is characterized by its rotation around its axis, while linear motion refers to the object's translational motion along a surface. Frictional forces can...
4.9K
Self-Presentation: Self-Monitoring and Self-Handicapping02:05

Self-Presentation: Self-Monitoring and Self-Handicapping

39.0K
People can go to great lengths to protect their self-image and present themselves in ways that they want others to see them. Sociologist Erving Goffman presented the idea that a person is like an actor on a stage. Calling his theory dramaturgy, Goffman believed that we use “impression management” to present ourselves to others as we hope to be perceived. Each situation is a new scene, and individuals perform different roles depending on who is present (Goffman, 1959). Think about...
39.0K
Rolling Without Slipping01:09

Rolling Without Slipping

3.6K
People have observed the rolling motion without slipping ever since the invention of the wheel. For example, one can look at the interaction between a car's tires and the surface of the road. If the driver presses the accelerator to the floor so that the tires spin without the car moving forward, there must be kinetic friction between the wheels and the road's surface. If the driver slowly presses the accelerator, causing the car to move forward, the tires roll without slipping. It is...
3.6K
Blinding01:11

Blinding

2.5K
Blinding is a commonly used method of not telling participants which treatment a subject is receiving. Blinding is a critical part of a randomized control trial or RCT. It reduces the bias that affects the results. In an RCT, blinding is used in the form of a placebo. A placebo effect occurs when untreated subjects falsely believe they have received the treatment and report improved symptoms. A placebo or a dummy treatment is administered to subjects to negate the bias caused by such an effect.
2.5K
Social Facilitation01:04

Social Facilitation

32.0K
Not all intergroup interactions lead to negative outcomes. Sometimes, being in a group situation can improve performance. Social facilitation occurs when an individual performs better when an audience is watching than when the individual performs the behavior alone. This typically occurs when people are performing a task for which they are skilled.
32.0K
Deindividuation00:57

Deindividuation

26.4K
Deindividuation is a form of social influence on an individual’s behavior such that the individual engages in unusual or non-normal behavior while in a group setting. Why? Because in these group settings, the individual no longer sees themselves as an individual anymore, disinhibiting their behavior and personal restraint.
26.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A unique poikilodermatous condition associated with a de novo heterozygous ATR missense variant.

Clinical and experimental dermatology·2026
Same author

Recessive loss of DIAPH1 function causes a progressive neurodevelopmental syndrome with variable immunological involvement.

Genetics in medicine : official journal of the American College of Medical Genetics·2026
Same author

Loss of CTLH component MAEA impairs DNA repair and replication and leads to developmental delay.

EMBO molecular medicine·2025
Same author

Identification of a Non-Coding Causative Variant Underlying Warsaw Breakage Syndrome Using Long-Read Based Genomic Sequencing and Transcriptome Analysis.

American journal of medical genetics. Part A·2025
Same author

The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication.

Nature communications·2025
Same author

USP37 prevents premature disassembly of stressed replisomes by TRAIP.

Nature communications·2025

Related Experiment Video

Updated: Jul 6, 2025

Scanning Light Scattering Profiler SLPS Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses
06:55

Scanning Light Scattering Profiler SLPS Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses

Published on: June 6, 2017

7.6K

DONSON: Slding in 2 the limelight.

Grant S Stewart1

  • 1Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.

DNA Repair
|December 30, 2023
PubMed
Summary
This summary is machine-generated.

The study identifies DONSON as the functional ortholog of yeast Sld2, crucial for DNA replication initiation and fork stability. This finding clarifies the role of DONSON in replication and may explain its link to neurodevelopmental disorders.

Keywords:
DONSONMeier-Gorlin syndrome, Replication stressMicrocephalic dwarfismReplication initiationSld2

More Related Videos

An Emerging Target Paradigm to Evoke Fast Visuomotor Responses on Human Upper Limb Muscles
09:27

An Emerging Target Paradigm to Evoke Fast Visuomotor Responses on Human Upper Limb Muscles

Published on: August 25, 2020

4.3K
Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
07:19

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

4.6K

Related Experiment Videos

Last Updated: Jul 6, 2025

Scanning Light Scattering Profiler SLPS Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses
06:55

Scanning Light Scattering Profiler SLPS Based Methodology to Quantitatively Evaluate Forward and Backward Light Scattering from Intraocular Lenses

Published on: June 6, 2017

7.6K
An Emerging Target Paradigm to Evoke Fast Visuomotor Responses on Human Upper Limb Muscles
09:27

An Emerging Target Paradigm to Evoke Fast Visuomotor Responses on Human Upper Limb Muscles

Published on: August 25, 2020

4.3K
Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
07:19

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

4.6K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Yeast replication initiation involves the pre-loading complex (pre-LC) including Sld2, Dpb11, GINS, and Polε.
  • Metazoan orthologs for Dpb11 (TOPBP1), Sld3 (TICRR), and Sld7 (MTBP) are known, but Sld2's ortholog remained controversial.
  • RECQL4 was previously proposed as the Sld2 ortholog due to limited homology, but lacked functional evidence for CMG loading.

Purpose of the Study:

  • To identify the functional vertebrate ortholog of yeast Sld2.
  • To elucidate the role of DONSON in DNA replication initiation and fork stability.
  • To understand the molecular basis of neurodevelopmental disorders linked to DONSON mutations.

Main Methods:

  • Comparative genomics to identify potential orthologs.
  • Biochemical assays to assess complex formation (DONSON, TOPBP1, GINS, Polε).
  • Functional studies investigating roles in DNA replication initiation and fork stability.

Main Results:

  • DONSON forms a complex with TOPBP1, GINS, and Polε, analogous to the yeast pre-LC.
  • This DONSON-containing complex is essential for positioning GINS on the MCM complex and initiating DNA replication.
  • DONSON demonstrates dual roles: promoting replication initiation and stabilizing replication forks during elongation.

Conclusions:

  • DONSON is identified as the functional ortholog of yeast Sld2 in metazoans.
  • DONSON plays critical roles in both DNA replication initiation and elongation.
  • These findings provide insights into the etiology of neurodevelopmental disorders associated with DONSON mutations.