Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A microcomputer program for determining turnover rates before and after intervention in a single animal.

P G Schmid, P G Schmid, C A Whiteis

    Computer Methods and Programs in Biomedicine
    |December 1, 1986
    PubMed
    Summary

    This study introduces three computer programs designed to calculate how quickly biomolecules are used and replaced in a single animal. These programs are based on a specific model of metabolism that assumes a steady-state relationship between a precursor and its product. The authors tested the programs in their lab using norepinephrine, a neurotransmitter, under various conditions. The programs can be adapted to study other substances that follow the same metabolic model, including neurotransmitters, peptides, and small molecules. The main benefit is the ability to study changes in biomolecule turnover in individual animals, which was previously difficult to achieve. The authors suggest this tool improves the accuracy of single-subject analysis in pharmacological and physiological research.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Reply from Y. Lu, C. A. Whiteis, K. A. Sluka, M. W. Chapleau and F. M. Abboud.

    The Journal of physiology·2013
    Same author

    Vascular and neural dysfunction in Zucker diabetic fatty rats: a difficult condition to reverse.

    Diabetes, obesity & metabolism·2007
    Same author

    Dual mechanisms of angiotensin-induced activation of mouse sympathetic neurones.

    The Journal of physiology·2006
    Same author

    Mechanisms of inducible nitric oxide synthase-mediated vascular dysfunction.

    Arteriosclerosis, thrombosis, and vascular biology·2005
    Same author

    Effect of fidarestat and alpha-lipoic acid on diabetes-induced epineurial arteriole vascular dysfunction.

    Experimental diabesity research·2004
    Same author

    Pharmacological effects of intravenous melatonin: comparative studies with thiopental and propofol.

    British journal of anaesthesia·2003

    Area of Science:

    • Computational biology
    • Pharmacokinetics modeling
    • Neurotransmitter metabolism

    Background:

    Prior research has established methods for measuring biomolecule turnover in controlled settings. However, no prior work had resolved how to apply these methods to single-animal studies with interventions. Existing techniques often require multiple animals or assume steady-state conditions. This gap motivated the development of a computational tool for single-subject analysis. It was already known that biomolecules follow specific metabolic models. Yet adapting these models to individual cases remained a challenge. The need for a flexible, single-animal approach became evident in pharmacological and physiological studies. This paper introduces a solution to that specific limitation.

    Purpose Of The Study:

    The aim of this work is to provide a computational framework for analyzing biomolecule turnover in single animals. The specific problem addressed is the lack of tools for single-subject kinetic analysis. This approach allows researchers to track changes before and after interventions. The motivation stems from the need to study physiological and pharmacological effects in individual models. Traditional methods require multiple subjects or assume steady-state conditions. This program removes those constraints by adapting to single-animal data. It was already known that many biomolecules follow open-compartment kinetics. The authors propose a solution that fits this model while allowing for individual variability.

    Keywords:
    biomolecule turnoversingle-compartment modelmetabolic analysiscomputational biology

    Frequently Asked Questions

    The programs calculate turnover rates based on a steady-state, open-compartment model. They determine values before and after an intervention in single animals.

    The programs can analyze biogenic amines, peptides, proteins, and small biological molecules that follow the model's constraints.

    The model allows the programs to calculate turnover rates accurately in single animals under varying conditions.

    MS-BASIC is the programming language used to develop the three programs for turnover analysis.

    Related Experiment Videos

    Main Methods:

    The authors developed three MS-BASIC programs to calculate turnover rates under specific kinetic models. These programs assume a steady-state precursor-product relationship. They also follow an open, single-compartment model for metabolism. The system determines turnover values before and after an intervention. The code was tested in a laboratory setting for norepinephrine turnover. The programs adapt to any substance following the model's constraints. It was already known that biogenic amines and peptides fit this model. The authors propose that the software can be applied to various biomolecules.

    Main Results:

    The programs successfully calculated turnover rates for norepinephrine in single animals. They were tested under differing physiological and pharmacological conditions. The results suggest the programs can detect changes caused by interventions. The system adapts to substances like neurotransmitters and small molecules. The authors propose that this tool improves single-subject analysis accuracy. It was already known that traditional methods require multiple subjects. This approach eliminates that need by using a computational model. The programs may be used for a wide range of biomolecules.

    Conclusions:

    The authors propose that these programs offer a flexible solution for single-animal turnover analysis. They suggest the tool can be applied to various substances following the model. The programs may improve the study of physiological and pharmacological effects. It was already known that traditional methods have limitations in single-subject studies. The authors propose that this system overcomes those limitations. The programs have been tested for norepinephrine turnover in laboratory settings. They may be adapted to other biomolecules with similar metabolic pathways. The authors suggest the tool is useful for a wide range of biological and pharmacological research.

    The programs were tested in a laboratory setting for norepinephrine turnover under physiological and pharmacological conditions.

    The authors suggest the programs improve single-subject analysis and may be applied to various biomolecules.