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Updated: Jul 6, 2025

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

189

TRAF4 regulates ubiquitination-modulated survivin turnover and confers radioresistance

Jinzhuang Liao1,2, Xiang Qing3, Xiaoying Li1,2

  • 1Department of Radiology, The Third Xiangya Hospital of Central South University, Changsha, 410013, Hunan, China.

International Journal of Biological Sciences
|January 2, 2024
PubMed

Insights

Tumor necrosis factor receptor-associated factor 4 (TRAF4) is overexpressed in nasopharyngeal carcinoma (NPC) and drives radioresistance. Targeting TRAF4 may enhance radiosensitivity in NPC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Nasopharyngeal carcinoma (NPC) recurrence after radiotherapy necessitates identifying resistance factors.
  • Tumor necrosis factor receptor-associated factor 4 (TRAF4) is a potential therapeutic target.

Purpose of the Study:

  • To investigate the role of TRAF4 in NPC radiosensitivity.
  • To elucidate the molecular mechanisms underlying TRAF4-mediated radioresistance.

Main Methods:

  • TRAF4 expression analysis in NPC cells and tissues.
  • TRAF4 knockdown experiments in vitro and in vivo.
  • Western blot analysis of the Akt/Wee1/CDK1/survivin axis.

Main Results:

  • TRAF4 is overexpressed in NPC and correlates with p-Akt and survivin.
  • TRAF4 knockdown enhances NPC radiosensitivity by inhibiting the Akt/Wee1/CDK1 axis.
  • TRAF4 knockdown suppresses survivin phosphorylation and promotes its degradation.
  • TRAF4 knockdown overcomes radioresistance in vitro and in xenograft models.

Conclusions:

  • The TRAF4-survivin axis is crucial for NPC radioresistance.
  • Targeting TRAF4 represents a promising strategy for radiosensitization in NPC treatment.
Keywords:
Nasopharyngeal carcinomaRadioresistanceSurvivinTRAF4Ubiquitination

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