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Updated: Jul 6, 2025

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

AptaDB: a comprehensive database integrating aptamer-target interactions.

Long Chen¹, Zhuohang Yu¹, Zengrui Wu¹

¹Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China.

RNA (New York, N.Y.)

|January 2, 2024

Summary

This summary is machine-generated.

A new database, AptaDB, integrates diverse aptamer data, including interactions and properties. This resource supports computational aptamer design and screening, advancing pharmacology and medicine.

Keywords:

annotation aptamer–target interaction database similar aptamers

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Last Updated: Jul 6, 2025

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Area of Science:

Biotechnology
Bioinformatics
Computational Biology

Background:

Aptamers show great potential in pharmacology, medicine, and analytical chemistry.
Lack of integrated data hinders computational aptamer development and reuse.
Existing aptamer databases lack comprehensive, validated information.

Purpose of the Study:

To develop a comprehensive, public-access database for aptamer-related data.
To facilitate computational methods and aptamer reuse.
To provide a user-friendly resource for aptamer research.

Main Methods:

Manually collected and integrated experimentally validated data from literature.
Included six key data components: aptamer-target interactions, properties, structures, targets, experimental activity, and similar aptamers.
Developed a user-friendly interface for data access.

Main Results:

AptaDB contains 1350 aptamer-target interactions, 1230 binding affinity constants, and 1293 aptamer sequences.
Features twice the number of entries compared to existing aptamer databases.
Unique integration of diverse aptamer data categories.

Conclusions:

AptaDB offers a unique and comprehensive resource for aptamer research.
The database is expected to be a powerful tool for rational aptamer design and screening.
AptaDB will be continuously updated to reflect advancements in aptamer research.