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MicroRNA-150-5P regulates Th1/Th2 cytokines expression levels by targeting EGR2 in allergic rhinitis

H Jian1, H Feng1, L Zhu1

  • 1Department of Otorhinolaryngology, the Third Affiliated Hospital of ZunYi Medical University/First People’s Hospital of Zunyi 563002, China.

Rhinology
|January 2, 2024
PubMed
Abstract

Insights

MicroRNA-150-5p (miR-150-5p) is upregulated in allergic rhinitis (AR), promoting T-cell differentiation via dendritic cells (DCs). This microRNA targets EGR2, influencing DC function and contributing to AR development.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-150-5p (miR-150-5p) is implicated in allergic rhinitis (AR) pathogenesis.
  • Previous studies suggest miR-150-5p plays a role in peripheral blood dendritic cells (DCs) of AR patients.

Purpose of the Study:

  • To investigate the role and mechanism of miR-150-5p in regulating DC function.
  • To explore how miR-150-5p modulates EGR2 and influences T cell differentiation in AR.

Main Methods:

  • Real-time quantitative polymerase chain reaction (qRT-PCR) for miR-150-5p and EGR2 expression.
  • Enzyme-linked immunosorbent assay (ELISA) for IL-4 cytokine levels.
  • HE staining for eosinophils and immunohistochemistry for EGR2 detection.

Main Results:

  • miR-150-5p was upregulated, while EGR2 was downregulated in peripheral blood DCs from AR patients.
  • miR-150-5p upregulated DCs, promoting T-cell differentiation.
  • miR-150-5p targeted EGR2, suppressing DCs and altering T-cell differentiation, thus triggering AR.

Conclusions:

  • MiR-150-5p and its target gene EGR2 are involved in AR development.
  • Dendritic cells in AR patients foster T-cell differentiation in peripheral blood.

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