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Peptide Identification Using Tandem Mass Spectrometry

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Updated: Jul 6, 2025

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis

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Accelerating Pharmaceutical Process Development with an Acoustic Droplet Ejection-Multiple Reaction Monitoring-Mass

Hang Hu¹, Andrew N Singh¹, Dan Lehnherr²

¹Analytical Research and Development, Merck & Co., Inc., Rahway, New Jersey 07065, United States.

Analytical Chemistry

|January 2, 2024

Summary

This summary is machine-generated.

Acoustic droplet ejection-multiple reaction monitoring-mass spectrometry (ADE-MRM-MS) accelerates pharmaceutical research by enabling rapid, sensitive, and selective nanoliter-scale measurements. This high-throughput workflow enhances reaction screening and catalyst development.

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Area of Science:

Analytical Chemistry
Chemical Engineering
Biotechnology

Background:

Pharmaceutical process development demands faster, more sensitive analytical assays for small sample volumes.
Acoustic droplet ejection (ADE) coupled with mass spectrometry offers high-throughput nanoliter measurements directly from microplates.

Purpose of the Study:

To introduce and validate an Acoustic Droplet Ejection-Multiple Reaction Monitoring-Mass Spectrometry (ADE-MRM-MS) workflow.
To accelerate pharmaceutical process research and development (PR&D) through enhanced analytical capabilities.

Main Methods:

Developed a systematic ADE-MRM-MS workflow for data-driven selection of MRM transitions and assay optimization.
Utilized rapid measurements (1 sample/s) for efficient parameter tuning.
Applied the workflow to screen Ni-based catalysts and enzyme libraries for specific reactions.

Main Results:

Achieved analytical assays with excellent sensitivity, selectivity, and speed for PR&D reaction screenings.
Successfully guided industrial processes including catalyst screening (1 Hz) and enzyme library screening.
Demonstrated ADE-MRM-MS assays provide accurate results comparable to conventional LC, with over 100-fold throughput increase.

Conclusions:

The ADE-MRM-MS workflow significantly enhances the speed and efficiency of pharmaceutical R&D.
This method enables high-throughput screening and optimization of chemical reactions and biological catalysts.
ADE-MRM-MS represents a powerful analytical tool for modern drug discovery and process development.