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Proteome dataset of Candida albicans (ATCC10231) opaque cell
Gajanan Zore1, Mazen Abdulghani2, Santosh Kodgire3
1Dept. of Biotechnology, School of Life Sciences, Central University of Rajasthan, Ajmer, Bandersindri NH 8, Kishangarh Dist Ajmer (Rajasthan), India.
Objectives:
Candida albicans, a polymorphic yeast, is one of the most common, opportunistic fungal pathogens of humans. Among the different morphological forms, opaque form is one of the least-studied ones. This opaque phenotype is essential for mating and is also reported to be involved in colonizing the gastrointestinal tract. Considering the significance of the clinical and sexual reproduction of C. albicans, we have investigated the morphophysiological modulations in opaque form using a proteomic approach.
Data Description:
In the current investigation, we have used Micro-Liquid Chromatography-Mass Spectrometry (LC-MS/MS) analysis to create a protein profile for opaque-specific proteins. Whole-cell proteins from C. albicans (ATCC10231) cells that had been cultured for seven days on synthetic complete dextrose (SCD) medium in both as an opaque (test) and as a white (control) form cells were extracted, digested, and identified using LC-MS/MS. This information is meant to serve the scientific community and represents the proteome profile (SWATH Spectral Libraries) of C. albicans opaque form.
Insights
This study profiles the proteome of the opaque form of Candida albicans, a yeast linked to infections and mating. Understanding these opaque-specific proteins aids research into fungal pathogenesis and reproduction.
Area of Science:
- Mycology
- Proteomics
- Molecular Biology
Background:
- Candida albicans is a common opportunistic human fungal pathogen.
- It exhibits various morphological forms, including the less-studied opaque form.
- The opaque phenotype is crucial for mating and gastrointestinal tract colonization.
Purpose of the Study:
- To investigate the morphophysiological modulations in the opaque form of Candida albicans.
- To characterize the proteome of the opaque form using a proteomic approach.
- To provide a proteomic profile for the scientific community.
Main Methods:
- Micro-Liquid Chromatography-Mass Spectrometry (LC-MS/MS) was employed.
- Whole-cell proteins were extracted from opaque and white forms of C. albicans (ATCC10231).
- Proteins were digested and identified using LC-MS/MS to generate a proteome profile (SWATH Spectral Libraries).
Main Results:
- A comprehensive protein profile of the opaque form of C. albicans was generated.
- Specific proteins associated with the opaque phenotype were identified.
- The study provides a valuable proteomic dataset for C. albicans opaque cells.
Conclusions:
- The proteomic analysis provides insights into the unique proteins of the opaque form.
- This data contributes to understanding the role of the opaque phenotype in C. albicans biology.
- The generated SWATH Spectral Libraries serve as a resource for future research on C. albicans opaque form.
