Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydrodynamic dispersion drives viral-cellular contact for gene delivery in porous media.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Nuclear SUN2 coordinates endothelial cell-matrix interactions to regulate blood vessel homeostasis and barrier function.

bioRxiv : the preprint server for biology·2026
Same author

A multimodal adaptive optical microscope for in vivo imaging from molecules to organisms.

Nature methods·2026
Same author

A series of spontaneously blinking dyes for super-resolution microscopy.

Nature methods·2026
Same author

Unstructured transcription factor interactions enable emergent specificity.

Science (New York, N.Y.)·2026
Same author

Measurement of cellular traction forces during confined migration.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Jul 6, 2025

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
09:24

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Published on: January 30, 2020

8.0K

Smart lattice light-sheet microscopy for imaging rare and complex cellular events.

Yu Shi1, Jimmy S Tabet1, Daniel E Milkie2

  • 1Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Nature Methods
|January 3, 2024
PubMed
Summary

Smart microscopy with artificial intelligence (AI) autonomously switches imaging modes to capture rare cell events. This enables detailed analysis of cell division and immune synapse formation with unprecedented speed and scale.

More Related Videos

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
08:42

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Published on: September 15, 2018

9.4K
Imaging Subcellular Structures in the Living Zebrafish Embryo
11:19

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

11.7K

Related Experiment Videos

Last Updated: Jul 6, 2025

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
09:24

Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy

Published on: January 30, 2020

8.0K
Light-sheet Fluorescence Microscopy for the Study of the Murine Heart
08:42

Light-sheet Fluorescence Microscopy for the Study of the Murine Heart

Published on: September 15, 2018

9.4K
Imaging Subcellular Structures in the Living Zebrafish Embryo
11:19

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

11.7K

Area of Science:

  • Cell Biology
  • Microscopy
  • Immunology

Background:

  • Biological processes occur across diverse spatiotemporal scales.
  • Capturing rare or transient events is challenging with single imaging modalities.
  • Long-term cellular phenotypes can originate from fleeting biological occurrences.

Purpose of the Study:

  • To develop an AI-driven microscope system for autonomous switching between imaging techniques.
  • To overcome limitations in capturing rare biological events.
  • To enable high-resolution, long-term imaging of complex cellular dynamics.

Main Methods:

  • Development of smartLLSM, a microscope integrating AI for instrument control.
  • Autonomous switching between epifluorescent inverted imaging and lattice light-sheet microscopy (LLSM).
  • Application to study cell division and immune synapse formation in heterogeneous cell populations.

Main Results:

  • smartLLSM provides population-level statistics across thousands of cells.
  • Autonomously captures multicolor 3D datasets and 4D time-lapse movies of rare events.
  • Quantified effects of Taxol on cell division and antigen strength on immune synapses.

Conclusions:

  • smartLLSM efficiently detects and records rare events in heterogeneous cell populations.
  • High spatiotemporal 4D imaging capabilities over statistically significant replicates.
  • Enables detailed analysis of cellular processes previously difficult to capture.