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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

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Updated: Jul 6, 2025

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

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Published on: July 5, 2019

Practical Considerations for Oncogenic Fusion Detection and Reporting in Solid Tumors.

James P Solomon¹

¹Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, United States.

The Journal of Applied Laboratory Medicine

|January 3, 2024

Summary

Oncogenic gene fusions in solid tumors are clinically significant. Next-generation sequencing, particularly RNA sequencing, offers advanced methods for detecting these fusions, improving oncologic diagnostics and treatment.

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Author Spotlight: FISH as a Tool for Precise Gene Amplification Assessment in Cancer Specimens

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Published on: July 12, 2024

Area of Science:

Oncology
Genomics
Molecular Diagnostics

Background:

Chromosomal rearrangements leading to oncogenic gene fusions are critical in solid tumors, impacting diagnosis and treatment.
Traditional methods like fluorescence in situ hybridization are being superseded by advanced genomic profiling techniques.

Purpose of the Study:

To review the evolution of gene fusion detection methods in oncology.
To discuss the advantages and disadvantages of various RNA sequencing platforms for fusion identification.
To emphasize the importance of systematic evaluation of RNA sequencing results for accurate fusion reporting.

Main Methods:

Comparison of traditional low-throughput methods with high-throughput next-generation sequencing (NGS) techniques.
Focus on RNA sequencing as a primary NGS method for comprehensive genomic profiling.
Evaluation of evidence (quality, structural, functional) for assessing identified fusion events.

Main Results:

RNA sequencing offers significant advantages over traditional methods for detecting gene fusions.
Multiple RNA sequencing platforms exist, each with unique benefits and limitations.
Systematic evaluation is crucial for reliable interpretation of fusion detection results.

Conclusions:

Understanding diverse fusion detection assays is vital for precision oncology.
Accurate identification and interpretation of gene fusions are essential for clinical decision-making.
The shift towards high-throughput sequencing methods enhances the capability to detect clinically significant gene fusions.