Acute Neuropixels recordings in the marmoset monkey

Area of Science:

• Neuroscience
• Systems Neuroscience
• Computational Neuroscience

Background:

• High-density neural probes like Neuropixels offer insights into neural population dynamics and behavior.
• Marmoset monkeys possess a smooth cortex ideal for perpendicular neural recordings and laminar computation studies.

Approach:

• A novel surgical method for acute Neuropixels recordings in the common marmoset (Callithrix jacchus).
• Utilizes an artificial silicon-based dura for improved cortical access, perpendicular probe insertion, and compatibility with optical techniques.
• The technique supports repeated acute penetrations over months, with potential for year-long recordings after minimal maintenance.

Key Points:

• Enables recording of hundreds of single neurons across cortical layers.
• Facilitates precise investigation of laminar computations and their behavioral relevance.
• Compatible with Neuropixels probes, semi-chronic microdrives, and wireless recording systems.

Conclusions:

• This method provides a robust platform for advanced neuroscience research in marmosets.
• It opens new avenues for understanding neural circuits and brain function at a laminar level.
• The technique is adaptable for various experimental paradigms, including combined optical and electrophysiological recordings.