Related Experiment Video
Updated: Jun 21, 2026
11:19
Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 Kir4.1
Published on: September 26, 2015
8.0K
Refinement of prognostication for IDH-mutant astrocytomas using DNA methylation-based classification
Teresia Kling1, Sandra Ferreyra Vega1, Medha Suman1
1Sahlgrenska Center for Cancer Research, Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Brain Pathology (Zurich, Switzerland)
|January 3, 2024
Summary
DNA methylation profiling accurately predicts outcomes for isocitrate dehydrogenase (IDH)-mutant astrocytomas, matching the World Health Organization (WHO) 2021 criteria. This molecular classification refines prognostication, especially for high-grade gliomas.
Area of Science:
- Neuro-oncology
- Molecular Pathology
- Genomics
Background:
- The 2021 World Health Organization (WHO) grading system for isocitrate dehydrogenase (IDH)-mutant astrocytomas integrates histological features and CDKN2A/B homozygous deletion status.
- DNA methylation profiling is increasingly vital for diagnosing central nervous system (CNS) tumors, including gliomas, and is adopted in clinical practice globally.
Purpose of the Study:
- To evaluate the prognostic value of DNA methylation-based classification in adult-type IDH-mutant astrocytomas.
- To compare the efficacy of methylation profiling against current WHO 2021 CNS criteria for patient prognostication.
Main Methods:
- Analysis of histopathological diagnoses, genome-wide DNA methylation array data, and chromosomal copy number alterations in 385 IDH-mutant astrocytomas.
- Assessment of prognosis using WHO 2021 CNS criteria, chromosomal/gene alterations, and DNA methylation subclassification via a molecular neuropathology classifier.
Main Results:
- DNA methylation-based classification demonstrated comparable prognostic accuracy to WHO 2021 CNS criteria for IDH-mutant astrocytomas.
- Methylation subclassification identified patients with poor survival within grade 3 and favorable outcomes within grade 4 IDH-mutant astrocytomas.
Conclusions:
- DNA methylation profiling offers significant prognostic information for IDH-mutant astrocytomas.
- Methylation-based subclassification can enhance the precision of the current WHO 2021 grading scheme for improved patient management.
Related Concept Videos
02:07Tumor Progression
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
02:07Tumor Progression
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...