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Related Experiment Videos

[Method of separating mouse platelets by discontinuous sucrose-gradient centrifugation].

M Tajima, H Tamura, K Maejima

    Jikken Dobutsu. Experimental Animals
    |January 1, 1987
    PubMed
    Summary

    Researchers separated mouse blood platelets into five distinct populations using a modified Booyse method. This study details the specific density distribution of these platelet populations in normal CD-1 mice.

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    (1-->3)-beta-D-glucan determination in rat organs with limulus coagulation factor G.

    Research in experimental medicine. Zeitschrift fur die gesamte experimentelle Medizin einschliesslich experimenteller Chirurgie·1997

    Area of Science:

    • Hematology
    • Biochemistry
    • Cell Biology

    Context:

    • Platelets are crucial for hemostasis and thrombosis.
    • Understanding platelet heterogeneity is essential for diagnosing and treating bleeding disorders.
    • Previous methods for platelet separation lacked sufficient resolution.

    Purpose:

    • To develop a refined method for separating mouse blood platelets.
    • To characterize the density distribution of distinct platelet populations in CD-1 mice.

    Summary:

    • A modified Booyse method utilizing discontinuous sucrose gradient centrifugation was employed.
    • Five distinct mouse platelet populations (A-E) were identified based on specific gravity.
    • The distribution of these populations in normal CD-1 mice was quantified, revealing specific density ranges and percentages.

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    Impact:

    • Provides a reproducible method for platelet population analysis in mice.
    • Establishes baseline data for platelet density distribution in a common mouse model.
    • Facilitates future research into platelet function and dysfunction.