Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coccidioidomycosis Exposure Assessed by Skin Testing and Environmental Factors in Baja California, Mexico.

Journal of fungi (Basel, Switzerland)·2026
Same author

Rapid and Efficient Antibody-Drug Conjugate Design Using Mechanistic Bottom-Up Modeling from In Vitro to Human.

Bioconjugate chemistry·2026
Same author

Influence of cations on nitrate-to-ammonia synthesis over NiO:SnO<sub>2</sub>: insights from differential electrochemical mass spectrometry.

Chemical communications (Cambridge, England)·2026
Same author

Targeting highly attenuated IL-18 to PD-1 for enhanced anti-tumor activity.

Frontiers in immunology·2026
Same author

A masking clamp for conditional activation of therapeutic antibodies.

Frontiers in immunology·2025
Same author

Covalent and Site-Specific Immobilization of a Fluorogenic Sensor Protein on Cellulose-Based Paper for Detection of Lactate in Cell Culture Media.

Biosensors·2025

Related Experiment Video

Updated: Jul 6, 2025

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line
06:38

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Published on: August 2, 2021

28.0K

3D topographies promote macrophage M2d-Subset differentiation.

Stefania C Carrara1,2, Amanda Davila-Lezama3,4, Clément Cabriel5

  • 1Institute for Organic Chemistry and Biochemistry, Technical University of Darmstadt, Alarich-Weiss-Strasse 4, D-64287 Darmstadt, Germany.

Materials Today. Bio
|January 3, 2024
PubMed
Summary

3D topographical substrates promote macrophage differentiation into M2d-like cells, crucial for drug discovery. This study demonstrates a novel method for in vitro cellular models without biochemical stimuli.

Keywords:
Fractal-like structuresIn vitro modelsMacrophage differentiationPhysical cuesStructured surfaceanti-inflammatory macrophages

More Related Videos

Polarization and Characterization of M1 and M2 Human Monocyte-Derived Macrophages on Implant Surfaces
08:37

Polarization and Characterization of M1 and M2 Human Monocyte-Derived Macrophages on Implant Surfaces

Published on: December 6, 2024

771
Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
09:32

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags

Published on: September 9, 2014

69.9K

Related Experiment Videos

Last Updated: Jul 6, 2025

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line
06:38

Macrophage Differentiation and Polarization into an M2-Like Phenotype using a Human Monocyte-Like THP-1 Leukemia Cell Line

Published on: August 2, 2021

28.0K
Polarization and Characterization of M1 and M2 Human Monocyte-Derived Macrophages on Implant Surfaces
08:37

Polarization and Characterization of M1 and M2 Human Monocyte-Derived Macrophages on Implant Surfaces

Published on: December 6, 2024

771
Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags
09:32

Isolation of Human Monocytes by Double Gradient Centrifugation and Their Differentiation to Macrophages in Teflon-coated Cell Culture Bags

Published on: September 9, 2014

69.9K

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Drug Discovery

Background:

  • In vitro cellular models are vital for drug discovery, but physiologically relevant systems are needed.
  • Current 2D and 3D culture techniques require improvement for mimicking in vivo conditions without external biochemical stimuli.
  • Physical cues, such as 3D topographical substrates, offer a novel approach to modulating cellular responses.

Purpose of the Study:

  • To investigate the differentiation of macrophage-like cells on 3D topographical inorganic substrates.
  • To analyze protein secretion and gene expression markers of macrophage subsets.
  • To explore the potential of 3D topographical surfaces for drug discovery applications.

Main Methods:

  • Culturing macrophage-like cells on 3D topographical inorganic substrates.
  • Analyzing protein secretion levels, specifically Interleukin-10 (IL-10).
  • Measuring mRNA expression of key macrophage subset markers, including IL-10 and Vascular Endothelial Growth Factor (VEGF).

Main Results:

  • Macrophages cultured on 3D topographical substrates differentiated into anti-inflammatory M2-type macrophages.
  • Increased secretion of IL-10 was observed in these differentiated macrophages compared to controls.
  • Upregulated IL-10 and VEGF mRNA indicated differentiation into the M2d subset, a key component of tumor-associated macrophages (TAMs).

Conclusions:

  • 3D topographical substrates can induce M2d macrophage differentiation, mimicking in vivo conditions.
  • This approach provides a physiologically relevant in vitro model for drug discovery without external biochemical stimulation.
  • Implementing 3D topographical inorganic surfaces can enhance the characterization of therapeutic modalities in drug discovery programs.