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Multiresponsive Core-Shell Microgels Functionalized by Nitrilotriacetic Acid.

Isabel K Sommerfeld1,2, Hanna Malyaran3,4,5, Sabine Neuss4,6

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Summary

We synthesized stimuli-responsive poly(VCL/NTAaa) microgels for drug delivery. These microgels show tunable responses to temperature, pH, and ionic strength, and can immobilize proteins like cytochrome c.

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Area of Science:

  • Materials Science
  • Polymer Chemistry
  • Biotechnology

Background:

  • Stimuli-responsive microgels with ionizable groups are valuable for applications like drug delivery and metal ion uptake.
  • Incorporating carboxylic groups enhances mucoadhesion, which is critical for targeted drug delivery systems.
  • Nitrilotriacetic acid (NTA)-functionalized microgels offer potential for specific molecular interactions but require detailed stimuli-responsive analysis.

Purpose of the Study:

  • To synthesize and characterize poly{N-vinylcaprolactam-2,2'-[(5-acrylamido-1-carboxypentyl)azanediyl]diacetic acid} [p(VCL/NTAaa)] microgels with varying NTA content.
  • To investigate the stimuli-responsive behavior of these microgels concerning temperature, ionic strength, and pH.
  • To explore potential applications, including cytocompatibility and protein immobilization.

Main Methods:

  • Precipitation polymerization for microgel synthesis.
  • Infrared (IR) spectroscopy, dynamic and electrophoretic light scattering for characterization.
  • NMR relaxometry, Atomic Force Microscopy (AFM), Scanning Electron Microscopy (SEM), and reaction calorimetry for morphological and responsive behavior analysis.

Main Results:

  • Successful synthesis of p(VCL/NTAaa) microgels with controlled NTA content.
  • Demonstrated responsiveness to temperature, ionic strength, and pH, addressing a gap in prior research.
  • Confirmed cytocompatibility and successful immobilization of cytochrome c, highlighting application potential.

Conclusions:

  • The synthesized p(VCL/NTAaa) microgels exhibit tunable stimuli-responsive properties.
  • These microgels are suitable for applications requiring controlled molecular interactions and drug delivery.
  • The study provides a foundation for further development of NTA-functionalized microgels in biomedical fields.