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Reference Intervals: A Hoffmann Method Improvement?

Dustin R Bunch1,2, Christopher D Koch3,4

  • 1Department of Pathology and Laboratory Medicine, Nationwide Children's Hospital, Columbus, OH, United States.

The Journal of Applied Laboratory Medicine
|January 3, 2024
PubMed
Abstract

No abstract available in PubMed .

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In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
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