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Related Concept Videos

Proteomics01:33

Proteomics

7.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses
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Liquid Biopsy Proteomics in Ophthalmology.

Julian Wolf1,2, Joel A Franco1,2, Rui Yip1,2

  • 1Molecular Surgery Laboratory, Stanford University, Palo Alto, California 94305, United States.

Journal of Proteome Research
|January 3, 2024
PubMed
Summary

Ocular liquid biopsies offer a minimally invasive way to analyze eye proteins, transforming diagnostics and disease understanding. This approach enables detailed molecular insights without damaging eye tissue, paving the way for personalized treatments.

Keywords:
aqueous humorartificial intelligenceeyeliquid biopsy proteomicsprecision medicinevitreous

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Area of Science:

  • Ophthalmology
  • Proteomics
  • Molecular Diagnostics

Background:

  • Minimally invasive ocular liquid biopsies offer a promising alternative to traditional tissue biopsies.
  • These biopsies capture locally enriched eye fluids containing proteins from specialized ocular cells.
  • Advantages include reduced functional damage, better tissue heterogeneity reflection, outpatient sampling, and therapeutic monitoring.

Purpose of the Study:

  • To review the opportunities, challenges, and future directions of high-resolution liquid biopsy proteomics in ophthalmology.
  • To highlight the potential of liquid biopsy proteomics for transforming molecular diagnostics, prognostics, and personalized medicine in eye diseases.
  • To introduce the TEMPO (Tracing Expression of Multiple Protein Origins) approach for examining disease mechanisms at the cellular level in vivo.

Main Methods:

  • Focus on large-scale collection of high-quality ocular fluid samples.
  • Emphasis on utilizing cutting-edge proteomics technologies for detailed protein analysis.
  • Integration of artificial intelligence-supported data analysis for interpreting complex proteomic data.

Main Results:

  • Liquid biopsies provide rich proteomic information from ocular fluids.
  • The TEMPO approach allows in-depth study of disease mechanisms in living patients.
  • High-resolution proteomics combined with AI can enhance diagnostic and prognostic capabilities.

Conclusions:

  • Ocular liquid biopsy proteomics holds significant potential to revolutionize ophthalmology.
  • It enables non-invasive disease monitoring, mechanistic studies, and personalized therapeutic strategies.
  • Advancements in sample collection, proteomics technology, and data analysis are crucial for realizing this potential.