Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Optimization of DNA collection card workflows on the RapidHIT™ ID instrument.

International journal of legal medicine·2026

Same author

Probabilistic searching of databases compared to binary CODIS eligibility: An improved methodology for maximizing investigative leads using short tandem repeat DNA profiles.

Forensic science international. Genetics·2026

Same author

Advancing forensic SNP typing: Insights from an interlaboratory study of the FORCE panel.

Forensic science international. Genetics·2026

Same author

Machine learning improves SNP microarray performance in challenged samples.

Bioinformatics advances·2026

Same author

Characterizing DNA mixtures with Demixtify.

Forensic science international. Genetics·2026

Same author

Bioinformatic processing of whole genome sequencing data with Tapir.

Forensic science international. Genetics·2025

Same journal

Comparison of key diagnostics for probabilistic interpretation of STR mixture data generated with length-based and MPS methodologies.

Forensic science international. Genetics·2026

Same journal

Likelihood Ratios Given Activity-Level Propositions for DNA Transfer Evidence: Theoretical Foundations of the HaloGen Framework (Part I).

Forensic science international. Genetics·2026

Same journal

Likelihood Ratios Given Activity-Level Propositions for DNA Transfer Evidence: Practical Implementation and Simulation Studies Using the HaloGen Engine (Part II).

Forensic science international. Genetics·2026

Same journal

Forensic evaluation of 101 identity-informative SNPs in a Filipino population using massively parallel sequencing.

Forensic science international. Genetics·2026

Same journal

Reprocessing of hair roots using conventional forensic DNA analysis following RapidHIT® ID processing.

Forensic science international. Genetics·2026

Same journal

Analysis of low-input and degraded forensic DNA trace samples with NGS using the MiSeq FGx platform.

Forensic science international. Genetics·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 6, 2025

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Published on: August 24, 2017

Identifying distant relatives using benchtop-scale sequencing.

August E Woerner¹, Nicole M Novroski², Sammed Mandape³

¹Center for Human Identification, University of North Texas Health Science Center, Fort Worth, TX, USA; Department of Microbiology, Immunology and Genetics, University of North Texas Health Science Center, Fort Worth, TX, USA.

Forensic Science International. Genetics

|January 3, 2024

Summary

This summary is machine-generated.

Forensic genetic genealogy (FGG) can now identify relatives up to 7th degree using low-pass sequencing on desktop sequencers. This cost-effective method enhances kinship analysis for identifying persons of interest.

Keywords:

Genetic genealogy Genotype imputation Kinship Low pass sequencing Massively parallel sequencing

More Related Videos

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Related Experiment Videos

Last Updated: Jul 6, 2025

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Detection of Rare Mutations in CtDNA Using Next Generation Sequencing

Published on: August 24, 2017

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

Methyl-binding DNA capture Sequencing for Patient Tissues

Methyl-binding DNA capture Sequencing for Patient Tissues

Published on: October 31, 2016

Area of Science:

Genetics
Forensic Science
Bioinformatics

Background:

Forensic genetic genealogy (FGG) uses genome-wide kinship estimates to identify individuals.
Current FGG methods are costly, requiring specialized equipment like microarrays or high-throughput sequencing.
Targeted PCR assays on common benchtop sequencers offer limited kinship resolution.

Purpose of the Study:

To evaluate low-pass sequencing as a cost-effective and accessible alternative for FGG.
To assess the resolution of low-pass sequencing for inferring kinship relationships.
To determine if desktop sequencers can achieve comparable kinship resolution to high-pass sequencing.

Main Methods:

Utilized low-pass whole genome sequencing on samples from a three-generation pedigree.
Augmented samples with simulated 7th-degree relatives.
Assessed kinship coefficient recovery using algorithms similar to those in GEDmatch.

Main Results:

Low-pass sequencing reliably inferred kinship up to 7th degree relatives.
The method is compatible with commonly available desktop sequencers.
Achieved resolutions comparable to high-pass sequencing methods.

Conclusions:

Low-pass sequencing is a viable and accessible strategy for forensic genetic genealogy.
This approach can significantly reduce costs associated with FGG.
Enables reliable identification of distant relatives using standard laboratory equipment.