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Published on: June 9, 2014
[Color Reaction Identification of Omphalotus guepiniformis Causing Food Poisoning]
Hideyuki Shinohara1, Ryuma Okawara1, Ikuko Ito1
1Yamagata Prefectural Institute of Public Health.
Abstract:
Simple identification using a color reaction was applied to investigate poisoning, putatively caused by Omphalotus guepiniformis. Some leftover uncooked mushrooms had turned turquoise green when a beam reagent (5 w/v% potassium hydroxide ethanolic solution) was dripped onto the mushroom pileus. Furthermore, ethanol extract of the mushrooms exhibited the same color reaction. Then, illudin S, a toxic compound contained in O. guepiniformis, was detected in uncooked leftover mushrooms using LC-MS/MS analysis. Therefore, this case was inferred as caused by O. guepiniformis. These results indicate the identification method described above as useful for screening tests for investigating food poisoning caused by O. guepiniformis.
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