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Related Concept Videos

Proteomics01:33

Proteomics

7.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Time-Lapse Epifluorescence Microscopy Imaging of Pseudomonas aeruginosa and Staphylococcus aureus Heterogeneous Phenotypes
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Proteomic characterization of persisters in Enterococcus faecium.

Charlotte Le Pont1, Benoît Bernay2, Mattéo Gérard1

  • 1UNICAEN, Univ Rouen Normandie, INSERM, DYNAMICURE UMR 1311, Caen, F-14000, France.

BMC Microbiology
|January 3, 2024
PubMed
Summary

The opportunistic pathogen Enterococcus faecium forms persister cells when exposed to ciprofloxacin, a survival mechanism potentially driving chronic infections and relapses. Proteomics identified key proteins involved in this stress response.

Keywords:
Enterococcus faeciumPersister cellsProteomicStress response

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Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Antimicrobial Resistance

Background:

  • Enterococcus faecium is a Gram-positive bacterium found in the gut microbiota.
  • It is also an opportunistic pathogen causing healthcare-associated infections.
  • Persister cells are a subpopulation that survives lethal conditions by arresting growth.

Purpose of the Study:

  • To investigate the formation of persister cells in Enterococcus faecium.
  • To identify molecular mechanisms underlying persister cell formation.

Main Methods:

  • Induction of persister cells using ciprofloxacin.
  • Kill curve analysis to assess bacterial survival.
  • Growth curves and antimicrobial susceptibility testing of persisters.
  • Genomic and comparative proteomic analyses.

Main Results:

  • Ciprofloxacin induced persister cell formation in E. faecium, characterized by biphasic kill curves.
  • Persisters were genotypically identical to wild-type cells but showed altered protein abundances.
  • Proteomic analysis revealed changes in proteins related to energy metabolism, transcription regulation, and stress response, including oxidative stress.

Conclusions:

  • Enterococcus faecium can enter a persister state, contributing to chronic infections and relapses.
  • Proteomic analysis identified potential key effectors of this phenotypical persister behavior.