Portable glucose sensing analysis based on laser-induced graphene composite electrode.

Area of Science:

• Electrochemistry
• Materials Science
• Biosensors

Background:

• Development of sensitive and portable glucose sensors is crucial for diabetes management and point-of-care diagnostics.
• Existing glucose sensing technologies face challenges in sensitivity, stability, and portability.

Purpose of the Study:

• To develop a portable electrochemical glucose sensor utilizing a novel laser-induced graphene (LIG) composite electrode.
• To enhance the sensor's performance through the integration of poly(3,4-ethylene dioxythiophene) (PEDOT) and gold nanoparticles (Au NPs).

Main Methods:

• Fabrication of a flexible graphene electrode via laser-induced graphene (LIG) technology.
• Potentiostatic deposition of PEDOT and Au NPs onto the LIG electrode to form a composite.
• Immobilization of glucose oxidase (GOx) using glutaraldehyde (GA) to create the sensing interface.
• Chronoamperometry was used for glucose concentration detection.

Main Results:

• The LIG/PEDOT/Au/GOx sensor detected glucose in a concentration range of 0.5 × 10^-5 to 2.5 × 10^-3 mol L^-1.
• Achieved high sensitivity of 341.67 μA mM^-1 cm^-2 and an ultra-low limit of detection of 0.2 × 10^-5 mol L^-1 (S/N = 3).
• Demonstrated excellent sensitivity, stability, and selectivity for biological fluid samples.

Conclusions:

• The developed LIG-based composite electrode offers a user-friendly platform for portable glucose sensing.
• The sensor's performance indicates suitability for integration into smartphone-based detection systems for point-of-care applications.
• Potential applications include environmental monitoring, public health surveillance, and food safety analysis.