Design and application of histidine-functionalized ZnCr-LDH nanozyme for promoting bacteria-infected wound healing

Excessive use of antibiotics can lead to an increase in antibiotic-resistant bacteria, which makes it a serious health threat. Therefore, developing new materials with antibacterial activity, such as nanozymes, has gained considerable attention. Reactive oxygen species (ROS) produced by nanozymes have rapid and effective antibacterial efficacy. Here, histidine (His) modified ZnCr layered double hydroxide (LDH) was synthesized inspired by the natural enzyme, and the enzyme-like activity of His/ZnCr-LDH was tested using a colorimetric method. Then, we developed an acid-enhanced antibacterial method based on the high peroxidase-like activity of His/ZnCr-LDH, and its ROS-generating capability in the presence of glucose oxidase (GOx) and glucose (Glu) as a source of hydrogen peroxide (H₂O₂). Gluconic acid (GA), the main product of the GOx reaction, provides an acidic environment and promotes ROS generation. The mentioned strategy shows high antibacterial activity at a low minimum inhibitory concentration (MIC) which represents the potential of His/ZnCr-LDH for effective bacterial elimination (3.5 μg mL^-1 for S. aureus and 6 μg mL^-1 for E. coli). In addition, animal experiments illustrated that the His/ZnCr-LDH can successfully boost the curing of infected wounds. The outcomes indicate that amino acid modified LDHs offer a new strategy for effective bacterial removal in different medical applications.