Related Experiment Video
Updated: Jul 6, 2025

Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products
Published on: March 12, 2020
Genome Mining Reveals the Biosynthesis of Sativene and Its Oxidative Conversion to seco-Sativene
Huaran Zhang1, Haiyan Zhao1, Yuqi Huang1
1College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, People's Republic of China.
Abstract:
Sativene (1) and seco-sativene are an important family of fungal sesquiterpenoids that feature unique tricyclo[4.4.0.01,7]decane and bicyclo[3.2.1]octane skeletons, respectively. Herein, we identify a three-enzyme cassette: SatA cyclizes farnesyl diphosphate (FPP) to form compound 1; CYP450 SatB catalyzes C14-C15 dihydroxylations and subsequent bond cleavage; and reductase SatC regioselectively reduces C14 aldehyde and mediates hemiacetal ring closure to generate prehelminthosporol (2). Our findings clarify the synthetic step of sativene and its oxidative transformation processes into seco-sativene.

